His Tag Antikörper

Produktnummer ABIN920801

Der Huhn Polyklonal Anti-His Tag-Antikörper wurde für WB und ELISA validiert. Er ist geeignet, His Tag in Proben von zu detektieren.

Kurzübersicht für His Tag Antikörper (ABIN920801)

Target: His Tag

Reaktivität: Bitte anfragen

Wirt: Huhn

Klonalität: Polyklonal

Konjugat: Dieser His Tag Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-His Tag Antikörper

Sequenz: HHHHHH

Aufreinigung: Affinity purified

Anwendungsinformationen

Applikationshinweise: Western blot: Use at 1:1,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: PBS pH 7.2 and 0.1% sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Details zu His Tag

Target: His Tag

Andere Bezeichnung: Hexahistidine-Tag

Substanzklasse: Tag

Hintergrund: Chickens were immunized with HHHHHH peptide conjugated to KLH. After multiple immunizations in Freund's adjuvant, eggs were collected and the immunoglobulin fraction prepared. Antibodies were immunoaffinity- purified using the peptide immobilized on a solid support. Specification: Antibody specificity was verified by ELISA against peptide conjugated to BSA (HHHHHH- BSA). A 1:8,000 dilution of the antibody gave an OD = 1.0 in a 15 minute reaction using HRP- conjugated goat anti-chicken IgY (IgG) at 1:10,000 at TMB as the substrate. Appropriate specificity controls were run.