PIDD Antikörper (AA 551-650) (Biotin)

Produktnummer ABIN905096

Der Kaninchen Polyklonal Anti-PIDD-Antikörper wurde für ELISA, IHC (p) und IHC (fro) validiert. Er ist geeignet, PIDD in Proben von Ratte zu detektieren.

Kurzübersicht für PIDD Antikörper (AA 551-650) (Biotin) (ABIN905096)

Target: PIDD (P53-Induced Death Domain Protein (PIDD))

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PIDD Antikörper ist konjugiert mit Biotin

Applikation: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-PIDD Antikörper (Biotin)

Bindungsspezifität: AA 551-650

Kreuzreaktivität: Ratte

Homologie: Human,Mouse

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human LRDD/PIDD

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: IHC-P 1:200-400
IHC-F 1:100-500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C for 12 months.

Haltbarkeit: 12 months

Details zu PIDD

Target: PIDD (P53-Induced Death Domain Protein (PIDD))

Andere Bezeichnung: LRDD

Hintergrund:

Synonyms: Leucine rich repeats and death domain containing, Leucine-rich repeats and death domain containing, MGC16925, p53-induced protein with a death domain, PIDD, DKFZp434D229, PIDD_HUMAN.

Background: The death domain (DD) containing protein PIDD is a p53 target gene in an erythroleukemia cell line that undergoes G1 phase arrest and subsequent apoptosis after p53 expression. Independently, PIDD was also described as a DD-containing protein with unknown function. The N-terminal region of PIDD contains seven leucine-rich repeats (LRRs), a protein interaction motif found in various proteins with diverse functions, followed by two ZU-5 domains and a C-terminal DD. PIDD forms a complex with caspase-2 and the adaptor protein RAIDD. Increased PIDD expression results in spontaneous activation of caspase-2 and sensitization to apoptosis by genotoxic stimuli, via interaction with caspase-2 and CRADD/RAIDD. PIDD also promotes apoptosis downstream of p53 as component of the DNA damage/stress response pathway that connects p53/TP53 to apoptosis. PIDD has also been shown to interact with NEMO/IKBKG and RIP1 and enhance sumoylation and ubiquitination of NEMO/IKBKG, an important component for activation of the transcription factor NF-kappa-B.

Pathways: p53 Signalweg, Caspase Kaskade in der Apoptose, Positive Regulation of Endopeptidase Activity