Cortactin Antikörper (pThr466) (AbBy Fluor® 350)

Produktnummer ABIN879745

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch Cortactin in WB, IF (cc) und IF (p). Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für Cortactin Antikörper (pThr466) (AbBy Fluor® 350) (ABIN879745)

Target: Cortactin (CTTN)

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Cortactin Antikörper ist konjugiert mit AbBy Fluor® 350

Applikation: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Produktdetails anti-Cortactin Antikörper (AbBy Fluor® 350)

Bindungsspezifität: pThr466

Kreuzreaktivität: Human

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human Cortactin around the phosphorylation site of Tyr466(YAp-TEA)

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu Cortactin

Target: Cortactin (CTTN)

Andere Bezeichnung: Cortactin

Hintergrund:

Synonyms: Amplaxin, CTTN, EMS 1, EMS1, Mammary tumor and squamous cell carcinoma associated, Oncogene EMS1, p80/85 src substrate, Src substrate cortactin, SRC8_HUMAN.

Background: Cortactin is an 80/85 kDa cytoskeleton protein that facilitates assembly of cortical actin. Cortactin is widely expressed in most adherent cells and is a prominent substrate of protein tyrosine kinase Src in vivo and in vitro. The protein sequence of cortactin contains multiple amino terminal tandem repeats of a unique 37 amino acid sequence, which associates with actin, and a Src homology 3 (SH3) domain at the carboxyl terminus. Between the repeat and the SH3 domain, there is an alpha helical structure followed by a proline rich region. Between the proline rich region and the SH3 domain, there are a series of Src phosphorylation sites (Tyr421, Tyr470, and Tyr486). In vitro, cortactin binds to and cross links F actin into meshworks.

Gen-ID: 2017

Pathways: MAPK Signalweg