PIDD Antikörper (AA 551-650)

Produktnummer ABIN873247

Dieser Kaninchen Polyklonal Antikörper erkennt spezifisch PIDD in ELISA, IF (cc), IF (p), IHC (p) und IHC (fro). Er zeigt eine Reaktivität gegenüber Ratte.

Kurzübersicht für PIDD Antikörper (AA 551-650) (ABIN873247)

Target: PIDD (P53-Induced Death Domain Protein (PIDD))

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PIDD Antikörper ist unkonjugiert

Applikation: ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-PIDD Antikörper

Bindungsspezifität: AA 551-650

Kreuzreaktivität: Ratte

Homologie: Human,Mouse

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human LRDD/PIDD

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit: 12 months

Details zu PIDD

Target: PIDD (P53-Induced Death Domain Protein (PIDD))

Andere Bezeichnung: LRDD

Hintergrund:

Synonyms: Leucine rich repeats and death domain containing, Leucine-rich repeats and death domain containing, MGC16925, p53-induced protein with a death domain, PIDD, DKFZp434D229, PIDD_HUMAN.

Background: The death domain (DD) containing protein PIDD is a p53 target gene in an erythroleukemia cell line that undergoes G1 phase arrest and subsequent apoptosis after p53 expression. Independently, PIDD was also described as a DD-containing protein with unknown function. The N-terminal region of PIDD contains seven leucine-rich repeats (LRRs), a protein interaction motif found in various proteins with diverse functions, followed by two ZU-5 domains and a C-terminal DD. PIDD forms a complex with caspase-2 and the adaptor protein RAIDD. Increased PIDD expression results in spontaneous activation of caspase-2 and sensitization to apoptosis by genotoxic stimuli, via interaction with caspase-2 and CRADD/RAIDD. PIDD also promotes apoptosis downstream of p53 as component of the DNA damage/stress response pathway that connects p53/TP53 to apoptosis. PIDD has also been shown to interact with NEMO/IKBKG and RIP1 and enhance sumoylation and ubiquitination of NEMO/IKBKG, an important component for activation of the transcription factor NF-kappa-B.

Pathways: p53 Signalweg, Caspase Kaskade in der Apoptose, Positive Regulation of Endopeptidase Activity