GAPDH Antikörper (AA 252-256)
Kurzübersicht für GAPDH Antikörper (AA 252-256) (ABIN871714)
Target
Alle GAPDH Antikörper anzeigenReaktivität
Wirt
Klonalität
Konjugat
Applikation
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Bindungsspezifität
- AA 252-256
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Spezifität
- The antibody detects endogenous level of total GAPDH protein.
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Aufreinigung
- Affinity purified
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Immunogen
- Peptide sequence around AA 252-256 (P-A-K-Y-D) derived from Human GAPDH. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.
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Applikationshinweise
- Western blotting: 1:500-1:1000
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Liquid
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Konzentration
- 1 mg/mL
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Buffer
- Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
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Konservierungsmittel
- Sodium azide
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Vorsichtsmaßnahmen
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Lagerung
- 4 °C/-20 °C
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Informationen zur Lagerung
- Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
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- GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))
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Andere Bezeichnung
- GAPDH
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Hintergrund
- Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC By similarity. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.
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Molekulargewicht
- 37 kDa
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Gen-ID
- 2597
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NCBI Accession
- NP_002037
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UniProt
- P04406
Target
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