GAPDH Antikörper (AA 136-335)

Produktnummer ABIN5518757

Zitiert in 115+ Publikationen. Der Kaninchen Polyklonal anti-GAPDH Antikörper (ABIN5518757) detektiert spezifisch GAPDH in WB, IHC, IF, ICC und FACS. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus, Ratte, Huhn, Affe und Zebrafisch (Danio rerio).

Kurzübersicht für GAPDH Antikörper (AA 136-335) (ABIN5518757)

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Reaktivität: Human, Maus, Ratte, Huhn, Affe, Zebrafisch (Danio rerio)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GAPDH Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Produktdetails anti-GAPDH Antikörper

Bindungsspezifität: AA 136-335

Verwendungszweck: Anti-GAPDH Antibody

Spezifität: No cross reactivity with other proteins.

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Homologie: Human GAPDH shares 95%,94.5% amino acid (aa) sequence identity with mouse,rat GAPDH,respectively.

Produktmerkmale: Anti-GAPDH Antibody. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat, Monkey, Chicken, Zebrafish.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human GAPDH recombinant protein (Position: N136-E335). Human GAPDH shares 95% and 94.5% amino acid (aa) sequence identity with mouse and rat GAPDH, respectively.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Human, Monkey, Mouse, Rat, Chicken, Zebrafish Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells, Human

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Referenzen für anti-GAPDH Antikörper (ABIN5518757)

Wang, Xue, Zhang, Bu, Zhu, Lai: "Autophagy protects ovarian cancer-associated fibroblasts against oxidative stress." in: Cell cycle (Georgetown, Tex.), Vol. 15, Issue 10, pp. 1376-85, (2018) (PubMed).

Duan, Man, Tang, Yao, Tao, Yu, Liang, Makawana, Zou, Wang, Zhu: "Design, Synthesis and Antitumor Activity of Novel link-bridge and B-Ring Modified Combretastatin A-4 (CA-4) Analogues as Potent Antitubulin Agents." in: Scientific reports, Vol. 6, pp. 25387, (2018) (PubMed).

Liu, Kuang, Wu, Jin, Sun: "A novel polysaccharide from Sargassum integerrimum induces apoptosis in A549 cells and prevents angiogensis in vitro and in vivo." in: Scientific reports, Vol. 6, pp. 26722, (2018) (PubMed).

Zou, Duan, Wang, Gao, Chen, Ou, Liang, Wang, Yuan, Wang, Zhu: "DYT-40, a novel synthetic 2-styryl-5-nitroimidazole derivative, blocks malignant glioblastoma growth and invasion by inhibiting AEG-1 and NF-κB signaling pathways." in: Scientific reports, Vol. 6, pp. 27331, (2018) (PubMed).

Guo, Li, Qian, Bi, He, Jin, Luo, Li, Meng, Li: "TGEV infection up-regulates FcRn expression via activation of NF-κB signaling." in: Scientific reports, Vol. 6, pp. 32154, (2018) (PubMed).

Li, Chen, Xiao, Li, Wang, Su, Guo, Liu, Yan, Yang, Zhang, Bu: "Mouse Sirt3 promotes autophagy in AngII-induced myocardial hypertrophy through the deacetylation of FoxO1." in: Oncotarget, Vol. 7, Issue 52, pp. 86648-86659, (2018) (PubMed).

Feng, Zhang, Zhou, Zhou, Li, Guo, Feng, Ma, Huang, Huang: "mir-218-2 promotes glioblastomas growth, invasion and drug resistance by targeting CDC27." in: Oncotarget, Vol. 8, Issue 4, pp. 6304-6318, (2018) (PubMed).

Li, Guan, Liu, Zhao, Li, Zhang, Tan, Mi, Li: "Epoxyeicosanoids prevent intervertebral disc degeneration in vitro and in vivo." in: Oncotarget, Vol. 8, Issue 3, pp. 3781-3797, (2018) (PubMed).

Hu, Cao, Pang, Luo, Zou, Feng, Li, Chen, Huang: "Tumor grade related expression of neuroglobin is negatively regulated by PPARγ and confers antioxidant activity in glioma progression." in: Redox biology, Vol. 12, pp. 682-689, (2018) (PubMed).

Liao, Xing, Xu, Liu, Zhang: "Evaluation of the circulating level of fibroblast activation protein α for diagnosis of esophageal squamous cell carcinoma." in: Oncotarget, Vol. 8, Issue 18, pp. 30050-30062, (2018) (PubMed).

Cai, Tan, Li, Gao, Li, Li, Tang, Li, Hui: "Glucagon-Like Peptide-1 (GLP-1) Treatment Ameliorates Cognitive Impairment by Attenuating Arc Expression in Type 2 Diabetic Rats." in: Medical science monitor : international medical journal of experimental and clinical research, Vol. 23, pp. 4334-4342, (2018) (PubMed).

Wu, Sun, Ye, Zhang, Guo, Huang, Jing, Xiang, Yu, Guo: "Iguratimod prevents ovariectomy‑induced bone loss and suppresses osteoclastogenesis via inhibition of peroxisome proliferator‑activated receptor‑γ." in: Molecular medicine reports, Vol. 16, Issue 6, pp. 8200-8208, (2018) (PubMed).

Liu, Liu, Tan, Tang, Long, Li, Liang, Chu, Yang: "Gaseous signalling molecule SO2 via Hippo‑MST pathway to improve myocardial fibrosis of diabetic rats." in: Molecular medicine reports, Vol. 16, Issue 6, pp. 8953-8963, (2018) (PubMed).

Wu, Xing, Zhang, Mao, Chen, Liang, Wang, Ren, Cui, Jiang, Wang, Zou, Ji: "Autophagy promotes fibrosis and apoptosis in the peritoneum during long-term peritoneal dialysis." in: Journal of cellular and molecular medicine, Vol. 22, Issue 2, pp. 1190-1201, (2018) (PubMed).

Gu, Fang, Kang, Hu, Yu, Li, Cheng, Gao: "Effect of ALDH2 on High Glucose-Induced Cardiac Fibroblast Oxidative Stress, Apoptosis, and Fibrosis." in: Oxidative medicine and cellular longevity, Vol. 2017, pp. 9257967, (2018) (PubMed).

Li, Wang, Qin, Fan, Tian, Liu: "Fingolimod suppresses neuronal autophagy through the mTOR/p70S6K pathway and alleviates ischemic brain damage in mice." in: PLoS ONE, Vol. 12, Issue 11, pp. e0188748, (2018) (PubMed).

Zou, Zhang, Yuan, Yang, Wu, Song, Zhu, Mao, Chen: "Endogenous hormone 2-methoxyestradiol suppresses venous hypertension-induced angiogenesis through up- and down-regulating p53 and id-1." in: Journal of cellular and molecular medicine, Vol. 22, Issue 2, pp. 957-967, (2018) (PubMed).

Huang, Pan, Mao, Zhang, Ma, Xi, You: "Sinapic Acid Inhibits the IL-1β-Induced Inflammation via MAPK Downregulation in Rat Chondrocytes." in: Inflammation, Vol. 41, Issue 2, pp. 562-568, (2018) (PubMed).

Miao, Margolis, Gao, Voss, Li, Martini, Norton, Bossé, Wankowicz, Cullen, Horak, Wind-Rotolo, Tracy, Giannakis, Hodi, Drake, Ball, Allaf, Snyder, Hellmann, Ho, Motzer, Signoretti, Kaelin, Choueiri et al.: "Genomic correlates of response to immune checkpoint therapies in clear cell renal cell carcinoma. ..." in: Science (New York, N.Y.), Vol. 359, Issue 6377, pp. 801-806, (2018) (PubMed).

Yang, Zhang, Li, Wen: "MALAT1 enhanced the proliferation of human osteoblasts treated with ultra‑high molecular weight polyethylene by targeting VEGF via miR‑22‑5p." in: International journal of molecular medicine, Vol. 41, Issue 3, pp. 1536-1546, (2018) (PubMed).

Details zu GAPDH

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Andere Bezeichnung: GAPDH

Hintergrund:

Background: Glyceraldehyde 3-phosphate dehydrogenase (abbreviated as GAPDH or less commonly as G3PDH) is an enzyme of ~37 kDa that catalyzes the sixth step of glycolysis and thus serves to break down glucose for energy and carbon molecules. This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. GAPDH is mapped to 12p13.31. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus.

Gene Full Name: glyceraldehyde-3-phosphate dehydrogenase

Molekulargewicht: 36 kDa

Gen-ID: 2597

UniProt: P04406