GRP78 Antikörper (N-Term)

Produktnummer ABIN863190

Produktdetails anti-GRP78 Antikörper

Target: GRP78 (HSPA5) (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5))

Bindungsspezifität: N-Term

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GRP78 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Spezifität: Detects ~78 kDa.

Kreuzreaktivität: Hund, Drosophila melanogaster, Hamster, Human, Maus, Ratte

Aufreinigung: Protein A Purified

Immunogen: Full length human GRP78 (Bip) his tagged at the N terminus

Anwendungsinformationen

Applikationshinweise:

• WB (1:2000)
• ICC/IF (1:100)
• optimal dilutions for assays should be determined by the user.

Kommentare:

0.5 μg/ml of ABIN863189 was sufficient for detection of Grp78 in 10 μg of rat tissue lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: -20°C

Referenzen für anti-GRP78 Antikörper (ABIN863190)

Griffiths, Del Bel, Wilk, Brill: "Cellular homeostasis in the Drosophila retina requires the lipid phosphatase Sac1." in: Molecular biology of the cell, Vol. 31, Issue 11, pp. 1183-1199, (2020) (PubMed).

Liu, Chowdhury, Fang, Liu, Srikant: "Attenuation of unfolded protein response and apoptosis by mReg2 induced GRP78 in mouse insulinoma cells." in: FEBS letters, Vol. 588, Issue 11, pp. 2016-24, (2014) (PubMed).

Coelho, Cairrão, Zeng, Pires, Coelho, Ron, Ryoo, Domingos: "Xbp1-independent Ire1 signaling is required for photoreceptor differentiation and rhabdomere morphogenesis in Drosophila." in: Cell reports, Vol. 5, Issue 3, pp. 791-801, (2013) (PubMed).

Details zu GRP78

Target: GRP78 (HSPA5) (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5))

Andere Bezeichnung: GRP78 (HSPA5 Produkte)

Hintergrund: GRP78 is a ubiquitously expressed, 78- kDa glucose regulated protein, and is commonly referred to as an immunoglobin chain binding protein (BiP). The BiP proteins are categorized as stress response proteins because they play an important role in the proper folding and assembly of nascent protein and in the scavenging of misfolded proteins in the endoplasmic reticulum lumen. Translation of BiP is directed by an internal ribosomal entry site (IRES) in the 5' non-translated region of the BiP mRNA. BiP IRES activity increases when cells are heat stressed (1). GRP78 is also critical for maintenance of cell homeostasis and the prevention of apoptosis (2). Luo et al. have provided findings that suggest GRP78 is essential for embryonic cell growth and pluripotent cell survival (3). In terms of diseases, GRP78 has been shown to be a reliable biomarker of hypoglycemia, to serve a neuroprotective function in neurons exposed to glutamate and oxidative stress (4), and its protein levels are reduced in the brains of Alzheimer's patients (5). Also, the induction of the GRP78 protein that results in severe glucose and oxygen deprivation could possible lead to drug resistance to anti-tumor drugs (6, 7).

Gen-ID: 3309

NCBI Accession: NP_005338

UniProt: P11021

Pathways: Thyroid Hormone Synthesis, ER-Nucleus Signaling