Rhodopsin Antikörper

Produktnummer ABIN863085

Der Maus Monoklonal Anti-Rhodopsin-Antikörper wurde für WB, IHC, ELISA, IP, ICC und IF validiert. Er ist geeignet, Rhodopsin in Proben von Rind (Kuh) zu detektieren. Es sind 2+ Publikationen verfügbar.

Kurzübersicht für Rhodopsin Antikörper (ABIN863085)

Target: Rhodopsin (RHO)

Reaktivität: Rind (Kuh)

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Rhodopsin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF)

Klon: 1D4

Produktdetails anti-Rhodopsin Antikörper

Spezifität: Detects ~40 kDa. Recognizes Rhodopsin (native and recombinant forms). No known reactivity to other proteins. Binds specifically to the C-terminal epitope -T-E-T-S-Q-V-A-P-A-(COOH).

Kreuzreaktivität: Vertebrate

Aufreinigung: Protein G Purified

Immunogen: Bovine Rhodopsin

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise:

• WB (1:1000)
• IHC (1:100)
• optimal dilutions for assays should be determined by the user.

Kommentare:

1 μg/ml of ABIN863084 was sufficient for detection of rhodopsin in 10 μg of rat eye lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: -20°C

Referenzen für anti-Rhodopsin Antikörper (ABIN863085)

Makowka, Bruegmann, Dusend, Malan, Beiert, Hesse, Fleischmann, Sasse: "Optogenetic stimulation of Gs-signaling in the heart with high spatio-temporal precision." in: Nature communications, Vol. 10, Issue 1, pp. 1281, (2019) (PubMed).

Cheng, Djajadi, Molday: "Cell-specific markers for the identification of retinal cells by immunofluorescence microscopy." in: Methods in molecular biology (Clifton, N.J.), Vol. 935, pp. 185-99, (2012) (PubMed).

Details zu Rhodopsin

Target: Rhodopsin (RHO)

Andere Bezeichnung: Rhodopsin

Substanzklasse: Chemical

Hintergrund: Rhodopsin consists of the protein moiety opsin and a reversibly covalently bound cofactor, retinal. Opsin, a bundle of seven membrane embedded alpha-helices, binds retinal, a photo reactive chromophore, in a central pocket (2, 3). In addition to being the pigment of the retina that is responsible for both the formation of the photoreceptor cells, its function is to specifically convey information stored in the specific geometry of the chormophore to the surface of the molecule upon light absorption (2). In the active state, rhodopsin activates transduction, a GTP binding protein. Once activated, transduction promotes the hydrolysis of cGMP by phosphodiesterase. Rhodopsin's activity is believed to be shut off by its phosphorylation followed by binding of the soluble protein arrestin (4). Mutations in the rhodopsin gene lead to retinitis pigmentosa, which can be inherited as an autosomal dominant, an autosomal recessive or an X-linked recessive disorder (5).

Gen-ID: 509933

NCBI Accession: NP_001014890

UniProt: P02699

Pathways: WNT Signalweg, Sensory Perception of Sound, Regulation of G-Protein Coupled Receptor Protein Signaling, Phototransduction