Notch1 Antikörper

Produktnummer ABIN807923

Es sind 4+ Publikationen für dieses Produkt verfügbar. Der Maus Monoklonal anti-Notch1 Antikörper wird verwendet zum Nachweis von Notch1 in Proben von Human und Maus. Er wurde validiert für WB, FACS, ICC, IP und IHC (fro).

Kurzübersicht für Notch1 Antikörper (ABIN807923)

Target: Notch1 (NOTCH1) (Notch 1 (NOTCH1))

Reaktivität: Human, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Notch1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: MN1A

Produktdetails anti-Notch1 Antikörper

Verwendungszweck: Anti-Notch1 Purified

Keine Kreuzreaktivität: Ratte

Kreuzreaktivität (Details): Human, Mouse

Produktmerkmale: The mouse monoclonal antibody mN1A recognizes intracellular domain of Notch 1 protein, mainly its activated form. The unprocessed Notch 1 protein is recognized with lower affinity.

Aufreinigung: Purified by protein-A affinity chromatography.

Reinheit: > 95 % (by SDS-PAGE)

Immunogen: GST fusion protein containing cdc10-NCR region of mouse Notch1

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise: Flow cytometry: Recommended dilution: 1-4 μg/mL. Intracellular staining.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Do not freeze.

Referenzen für anti-Notch1 Antikörper (ABIN807923)

Khwaja, Liu, Tong, Jin, Pear, van Deursen, Bram: "HIV-1 Rev-binding protein accelerates cellular uptake of iron to drive Notch-induced T cell leukemogenesis in mice." in: The Journal of clinical investigation, Vol. 120, Issue 7, pp. 2537-48, (2010) (PubMed).

Watanabe, Nagaoka, Lee, Bianco, Gonzales, Castro, Rangel, Sakamoto, Sun, Callahan, Salomon: "Enhancement of Notch receptor maturation and signaling sensitivity by Cripto-1." in: The Journal of cell biology, Vol. 187, Issue 3, pp. 343-53, (2009) (PubMed).

Sun, Li, Vercherat, Gulbagci, Acharjee, Li, Chung, Thin, Taneja: "Stra13 regulates satellite cell activation by antagonizing Notch signaling." in: The Journal of cell biology, Vol. 177, Issue 4, pp. 647-57, (2007) (PubMed).

Huppert, Le, Schroeter, Mumm, Saxena, Milner, Kopan: "Embryonic lethality in mice homozygous for a processing-deficient allele of Notch1." in: Nature, Vol. 405, Issue 6789, pp. 966-70, (2000) (PubMed).

Details zu Notch1

Target: Notch1 (NOTCH1) (Notch 1 (NOTCH1))

Andere Bezeichnung: Notch1

Hintergrund: Notch receptor 1,Notch 1 is a 270-300 kDa transmembrane heterodimeric protein with multiple extracellular growth factor-like repeats, and with an intracellular domain consisting of multiple different domain types. It serves as a receptor for membrane ligands, such as Delta 1, Jagged 1 (CD339), and Jagged 2, and regulates cell fate decisions. Upon ligand binding the transmembrane form of Notch 1 is repeatedly cleaved to provide approximately 120 kDa Notch intracellular fragment (NICD), which translocates to the nucleus and acts as a part of transcriptional complexes that alter differentiation, proliferation, and apoptosis. The highest level of Notch 1 expression is in brain, lung and thymus.,AOS5, TAN1, hN1, AOVD1

Gen-ID: 4851

UniProt: P46531

Pathways: Notch Signalweg, Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development