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Notch1 Antikörper (AA 20-216) (APC)

NOTCH1 Reaktivität: Maus WB, IF, ICC Wirt: Maus Monoclonal S253-32 APC
Produktnummer ABIN2483198
  • Target Alle Notch1 (NOTCH1) Antikörper anzeigen
    Notch1 (NOTCH1) (Notch 1 (NOTCH1))
    Bindungsspezifität
    • 16
    • 16
    • 13
    • 11
    • 7
    • 5
    • 5
    • 5
    • 4
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 20-216
    Reaktivität
    • 118
    • 93
    • 49
    • 9
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Maus
    Wirt
    • 94
    • 41
    • 6
    • 4
    • 3
    • 2
    • 1
    Maus
    Klonalität
    • 89
    • 58
    • 2
    Monoklonal
    Konjugat
    • 70
    • 11
    • 9
    • 9
    • 7
    • 6
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser Notch1 Antikörper ist konjugiert mit APC
    Applikation
    • 95
    • 52
    • 52
    • 32
    • 27
    • 26
    • 23
    • 20
    • 15
    • 11
    • 10
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)
    Spezifität
    Detects >270 kDa. Does not cross-react with Notch 2 or Notch3.
    Kreuzreaktivität
    Human, Maus, Ratte
    Aufreinigung
    Protein G Purified
    Immunogen
    Fusion protein amino acids 20-216 (extracellular N-terminus, EGF-like domains 1-5) of mouse Notch1
    Klon
    S253-32
    Isotyp
    IgG1
    Top Product
    Discover our top product NOTCH1 Primärantikörper
  • Applikationshinweise
    • WB (1:1000)
    • optimal dilutions for assays should be determined by the user.
    Kommentare

    1 μg/ml of ABIN2483198 was sufficient for detection of Notch1 in 20 μg of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    1 mg/mL
    Buffer
    PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C
    Informationen zur Lagerung
    Conjugated antibodies should be stored at 4°C
  • Target
    Notch1 (NOTCH1) (Notch 1 (NOTCH1))
    Andere Bezeichnung
    Notch1 (NOTCH1 Produkte)
    Synonyme
    notch1 antikoerper, NOTCH1 antikoerper, hn1 antikoerper, tan1 antikoerper, notch antikoerper, xotch antikoerper, xnotch antikoerper, notch-1 antikoerper, xnotch1 antikoerper, TAN1 antikoerper, hN1 antikoerper, 9930111A19Rik antikoerper, Mis6 antikoerper, N1 antikoerper, Tan1 antikoerper, lin-12 antikoerper, NOTCH antikoerper, Xotch antikoerper, notch1-a antikoerper, notch 1 antikoerper, fibropellin-1 antikoerper, Notch homolog 1, translocation-associated (Drosophila) antikoerper, Notch homolog 1, translocation-associated antikoerper, notch 1 S homeolog antikoerper, notch1 antikoerper, LOC100631526 antikoerper, NOTCH1 antikoerper, Notch1 antikoerper, notch1.S antikoerper
    Hintergrund
    Notch is synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase (S1 cleavage) in the trans-golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase (S3 cleavage) to release the intracellular domain (NICD) from the membrane.
    Gen-ID
    18128
    NCBI Accession
    NP_032740
    UniProt
    Q01705
    Pathways
    Notch Signalweg, Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development
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