MAP2K5 Antikörper (pSer311, pThr315) (HRP)

Produktnummer ABIN801957

Dieses Anti-MAP2K5-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von MAP2K5 in WB, ELISA, IHC (p) und IHC (fro). Geeignet für Maus.

Kurzübersicht für MAP2K5 Antikörper (pSer311, pThr315) (HRP) (ABIN801957)

Target: MAP2K5 (Mitogen-Activated Protein Kinase Kinase 5 (MAP2K5))

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MAP2K5 Antikörper ist konjugiert mit HRP

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-MAP2K5 Antikörper (HRP)

Bindungsspezifität: pSer311, pThr315

Kreuzreaktivität: Maus

Homologie: Human,Rat,Dog,Cow,Sheep,Pig,Horse,Chicken,Rabbit

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human MEK5 around the phosphorylation site of Ser311+Thr315

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handhabung: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu MAP2K5

Target: MAP2K5 (Mitogen-Activated Protein Kinase Kinase 5 (MAP2K5))

Andere Bezeichnung: MEK5+

Hintergrund:

Synonyms: MAP2K5 phospho S311/T315, Dual specicity mitogen activated protein kinase kinase 5, MAP kinase kinase 5, MAP kinase kinase MEK5b, MAP2K5, MAPK/ERK kinase 5, MAPKK5, PRKMK5, EC 2.7.12.2.

Background: MEK5 is a dual specificity protein kinase belonging to thr Ser/Thr protein kinase family, (MAP kinase kinase family). It is activated by phosphorylation on Ser/Thr by MAP kinase kinases and interacts specifically with ERK5, and not with another MAP kinase like P38. This kinase specifically interacts with and activates MAPK7/ERK5. This kinase itself can be phosphorylated and activated by MAP3K3/MEKK3, as well as by atypical protein kinase C isoforms (aPKCs). It is not phosphorylated by RAFA, RAFB or RAFC and it may interact with GTPases such as CDC42. The signal cascade mediated by this kinase is involved in growth factor stimulated cell proliferation and muscle cell differentiation. MEK5 is expressed in many adult tissues and is most abundant in heart and skeletal muscle.

Gen-ID: 5607

UniProt: Q13163

Pathways: MAPK Signalweg, Neurotrophin Signalübertragung