PDGFRA Antikörper (AA 1035-1053)

Produktnummer ABIN790499

Der Kaninchen Polyklonal Anti-PDGFRA-Antikörper wurde für WB, IHC, IHC (p) und IP validiert. Er ist geeignet, PDGFRA in Proben von Human, Maus, Ratte, Hund, Rind (Kuh), Huhn, Affe, Schwein, Kaninchen, Pferd, Xenopus laevis, Fledermaus und Hamster zu detektieren. Es ist 1 Publikation verfügbar.

Kurzübersicht für PDGFRA Antikörper (AA 1035-1053) (ABIN790499)

Target: PDGFRA (Platelet Derived Growth Factor Receptor alpha (PDGFRA))

Reaktivität: Human, Maus, Ratte, Hund, Rind (Kuh), Huhn, Affe, Schwein, Kaninchen, Pferd, Xenopus laevis, Fledermaus, Hamster

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PDGFRA Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Produktdetails anti-PDGFRA Antikörper

Bindungsspezifität: AA 1035-1053

Marke: IHC-plus™

Spezifität: Recognizes human PDGF Receptor Type A, doublet, Mr 150/170kD. Species Crossreactivity: mouse. Species Sequence Homology: frog, chicken and zebra fish.

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Bat, Dog, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Lizard, Xenopus, Pufferfish (100%) Stickleback (95%) Zebrafish (89%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide corresponding to amino acids 1035-1053 (C-GKRNRHSSQTSEESAIETG) of human PDGF (KLH-conjugated). Receptor alpha. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Bat, Dog, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Lizard, Xenopus, Pufferfish (100%), Stickleback (95%), Zebrafish (89%).

Type of Immunogen: Synthetic peptide - KLH conjugated

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (1:100), IP, WB (1:250 - 1:1000)

Usage: Suitable for use in Western Blot and Immunoprecipitation. Western Blot: 1:250-1:1000 dilution detected PDGF Receptor a doublet (150/170kD) in 3T3/NIH cell lysate. 3T3/NIH cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-PDGF Receptor a (1:500 dilution). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: 5-10 μL immunoprecipitates PDGF Receptor a from 500 μg of 3T3/NIH RIPA lysate.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: 0.1 M Tris-glycine,  pH 7.4, 150 mM sodium chloride, 0.05 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C

Informationen zur Lagerung: May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use.

Referenzen für anti-PDGFRA Antikörper (ABIN790499)

Nance, Shi, Hakim, Wasala, Yue, Pan, Zhang, Robinson, Duan, Yao, Yang, Chen, Wagner, Gersbach, Duan: "AAV9 Edits Muscle Stem Cells in Normal and Dystrophic Adult Mice." in: Molecular therapy : the journal of the American Society of Gene Therapy, Vol. 27, Issue 9, pp. 1568-1585, (2019) (PubMed).

Details zu PDGFRA

Target: PDGFRA (Platelet Derived Growth Factor Receptor alpha (PDGFRA))

Andere Bezeichnung: PDGFRA / PDGFR Alpha

Hintergrund: Name/Gene ID: PDGFRA
Subfamily: PDGF Receptor
Family: Protein Kinase

Synonyms: PDGFRA, CD140A, CD140a antigen, PDGFalphaR, PDGFR2, PDGFRalpha, AlphaPDGFR, RHEPDGFRA, PDGF receptor alpha, PDGFR-2, PDGFRA/BCR fusion, APDGFR, Pdgf alpha-receptor, PDGF-R-alpha, PDGFR-alpha

Gen-ID: 5156

UniProt: P16234

Pathways: RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Platelet-derived growth Factor Receptor Signaling