SKA1 Antikörper (C-Term)

Produktnummer ABIN783203

Der Kaninchen Polyklonal anti-SKA1 Antikörper wird verwendet zum Nachweis von SKA1 in Proben von Human, Maus und Ratte. Er wurde validiert für WB, IHC (p) und EIA.

Kurzübersicht für SKA1 Antikörper (C-Term) (ABIN783203)

Target: SKA1 (Spindle and Kinetochore Associated Complex Subunit 1 (SKA1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SKA1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Produktdetails anti-SKA1 Antikörper

Bindungsspezifität: C-Term

Spezifität: This antibody recognizes the C-term of SKA1.

Kreuzreaktivität (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Aufreinigung: Immunoaffinity Chromatography

Immunogen: Synthetic peptide - KLH conjugated. Antigen Modification: C-Terminus Genename: SKA1

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS containing 0.02 % Sodium Azide as preservative

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid freeze-thaw cycles.

Details zu SKA1

Target: SKA1 (Spindle and Kinetochore Associated Complex Subunit 1 (SKA1))

Andere Bezeichnung: SKA1

Hintergrund: Upon entry into mitosis, the cell's microtubule (MT) network forms the mitotic spindle, allowing the segregation of paired chromosomes. Proteinaceous structures on centromeric chromatin termed kinetochores (KT) are essential for the proper attachment of the chromosomes to the spindle MTs. A recently discovered spindle and kinetochore complex, comprised of proteins SKA1, SKA2, and SKA3, has been found to be required for stable KT-MT interactions and timely anaphase onset. Depletion of either SKA1 or SKA2 by siRNA results in the loss of both proteins from the KT, but does not impact overall KT structure. Cells depleted of the SKA complex undergo a prolonged checkpoint-dependent delay in a metaphase-like state, indicating the importance of the SKA complex in the maintenance of the metaphase plate and spindle checkpoint silencing.Synonyms: C18orf24, Spindle and kinetochore-associated protein 1

Gen-ID: 220134

NCBI Accession: NP_001034624

UniProt: Q96BD8

Pathways: M Phase