ASAH1 Antikörper (AA 25-125)

Produktnummer ABIN782337

Dieses Anti-ASAH1-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von ASAH1 in WB, IHC (p) und EIA. Geeignet für Human.

Kurzübersicht für ASAH1 Antikörper (AA 25-125) (ABIN782337)

Target: ASAH1 (N-Acylsphingosine Amidohydrolase (Acid Ceramidase) 1 (ASAH1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ASAH1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Klon: 1A7

Produktdetails anti-ASAH1 Antikörper

Bindungsspezifität: AA 25-125

Spezifität: Recognizes Acid ceramidase ASAH1.

Kreuzreaktivität (Details): Species reactivity (tested):Human.

Aufreinigung: Protein A Chromatography

Immunogen: ASAH1 antibody was raised against ASAH1 (NP_808592, 25 a.a. ~ 125 a.a) partial recombinant protein with GST tag.

Isotyp: IgG3

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: PBS, pH 7.2

Handhabung: Avoid freeze-thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: Store the antibody (in aliquots) at -20° or -80 °C.

Details zu ASAH1

Target: ASAH1 (N-Acylsphingosine Amidohydrolase (Acid Ceramidase) 1 (ASAH1))

Andere Bezeichnung: Acid Ceramidase

Hintergrund: Sphingolipids are hydrolyzed by ceramidases to yield sphingosine and fatty acids. These ceramidases are classified according to the pH range that supports their optimal activity. ASAH1 is an acid ceramidase and key regulator of ceramide metabolism. Mutations in this gene results in Farber Lipogranulomatosis, a fatal human genetic disorder that results in the painful swelling of the joints and tendons and pulminary insufficiency, while a complete knockout of its expression is lethal in mice. Recent studies have shown elevated levels of ASAH1 in Alzheimer's disease (AD) patients correlating with a reduction in sphingomyelin and elevation of ceramide. Pretreatment of cultured neurons with recombinant AHAH1 prevented the cells from undergoing A-beta (Ab)-induced apoptosis. Multiple isoforms of this protein are known to exist.Synonyms: ASAH, ASAH1, Acylsphingosine deacylase, N-acylsphingosine amidohydrolase, PHP32, Putative 32 kDa heart protein

Gen-ID: 427

NCBI Accession: NP_001120977

UniProt: Q13510