Keratin Basic Antikörper

Produktnummer ABIN768698

Der Maus Monoklonal anti-Keratin Basic Antikörper wird verwendet zum Nachweis von Keratin Basic in Proben von Human. Er wurde validiert für WB, IHC (p), IHC und IHC (fro).

Kurzübersicht für Keratin Basic Antikörper (ABIN768698)

Target: Keratin Basic

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: AE3

Produktdetails

Spezifität: This antibody has shown to recognize most of the basic (type II) keratins. Each epithelium contains at least one basic keratin. In this way AE-3 is broadly reactive with almost all epithelial and there neoplasms. This antibody together with AE-1 has been used for studying the immuno-localization of epidermal keratins, tissue distribution and epithelia development. AE-1 and AE-3 have also been used extensively, individually or mixed, for distinguishing carcinoma from non-carcinoma. The antibody is cross-reactive with keratins of rabbit, mouse, rat, bovine and chicken. Within immunoblots AE-1 detects distinct MW's of 52 kD, 54 kD, 56 kD, 58 kD, 59 kD, 64 kD, and 65-67 kD with all Ip's lower then 6.0. These MW's are believed to correspond with respectively keratin 8, 7, 6, 5, 4, 3 and 1 & 2.

Aufreinigung: Purified IgG

Immunogen: Human epidermal cells.

Type of Immunogen: Cells

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-Fr, IHC-P (1:100), WB (1:100)

Usage: In unfixed frozen sections and formalin-fixed paraffin embedded tissues. Also applicable in Western blotting. For use on formalin-fixed paraffin embedded tissues a dilution of approximately 1:150 to 1:200 is appropriate if section are incubated for 30 min. at 37 °C. Possibly, higher dilutions can be used if sections are incubated at 4 °C overnight. For use in Western blotting a dilution of 1:100 is advised.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: 0.01M PBS containing 0.1 % Sodium azide and 1 % BSA. Sourced in Ascites.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 4°C.

Antigendetails

Target: Keratin Basic

Andere Bezeichnung: Basic Cytokeratin