Angiopoietin 1 Antikörper (Internal Region)

Produktnummer ABIN768552

Dieses Anti-Angiopoietin 1-Antikörper ist ein Ziege Polyklonal-Antikörper zur Detektion von Angiopoietin 1 in WB, ELISA, FACS und IF. Geeignet für Human.

Kurzübersicht für Angiopoietin 1 Antikörper (Internal Region) (ABIN768552)

Target: Angiopoietin 1 (ANGPT1)

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Angiopoietin 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)

Güteklasse: Verified

Produktdetails anti-Angiopoietin 1 Antikörper

Bindungsspezifität: Internal Region

Verwendungszweck: ANGPT1

Sequenz: PDFSSQKLQH

Kreuzreaktivität: Rind (Kuh), Hund, Human, Maus, Schwein, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-PDFSSQKLQH, from the internal region of the protein sequence according to NP_001137.2.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western Blot: In transfected HEK293 transiently expressing full-length Human ANGPT1 (myc and DYKDDDDK tagged), a band of approx. 48 kDa was observed. No bands were observed in mock-transfected HEK293 and the 48 kDa plus a 75 kDa band was observed using anti

Peptide ELISA: antibody detection limit dilution 1:32000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the plasma membranes of A431 and HeLa cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentr

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu Angiopoietin 1

Target: Angiopoietin 1 (ANGPT1)

Andere Bezeichnung: ANGPT1

Hintergrund: ANGPT1, angiopoietin 1, AGP1, AGPT, ANG1

Molekulargewicht: 48kDa

Gen-ID: 284, 11600, 89807

NCBI Accession: NP_001137

Pathways: RTK Signalweg, Glycosaminoglycan Metabolic Process