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WNT2 Antikörper (AA 221-320)

WNT2 Reaktivität: Human, Maus WB, ELISA Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN762896
  • Target Alle WNT2 Antikörper anzeigen
    WNT2 (Wingless-Type MMTV Integration Site Family Member 2 (WNT2))
    Bindungsspezifität
    • 14
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 221-320
    Reaktivität
    • 30
    • 21
    • 6
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    Human, Maus
    Wirt
    • 29
    • 1
    Kaninchen
    Klonalität
    • 29
    • 1
    Polyklonal
    Konjugat
    • 16
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser WNT2 Antikörper ist unkonjugiert
    Applikation
    • 29
    • 5
    • 5
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA
    Kreuzreaktivität
    Human, Maus
    Aufreinigung
    Purified by Protein A.
    Immunogen
    KLH conjugated synthetic peptide derived from human WNT2
    Isotyp
    IgG
  • Applikationshinweise
    WB 1:300-5000
    ELISA 1:500-1000
    IHC-P 1:200-400
    IHC-F 1:100-500
    IF(IHC-P) 1:50-200
    IF(IHC-F) 1:50-200
    IF(ICC) 1:50-200
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Validierung #029629 (Immunohistochemistry)
    'Independent Validation' Siegel
    by
    Histopathology and Tissue Shared Resource, Georgetown Lombardi Comprehensive Cancer Center
    No.
    #029629
    Datum
    16.03.2014
    Antigen
    Chargennummer
    131105
    Validierte Anwendung
    Immunohistochemistry
    Positivkontrolle
    Human glioma tissue
    Negativkontrolle
    Human brain
    Bewertung
    Signal was detected in positive control tissue and not in negative control tissue.
    'Independent Validation' Siegel
    Validierungsbilder
    Protokoll
    Primärantikörper
    • Antigen: Wingless-Type MMTV Integration Site Family Member 2 (WNT2) (N-Term)
    • Catalog number: ABIN762896
    • Lot number: 131105
    Sekundärantikörper
    • Antibody: Envision Plus Horse Radish Peroxidase conjugated anti-rabbit antibody
    • Batch number: 10082183
    Full Protocol
    • Immunohistochemistry was performed by hand.
    • Sections were de-paraffinized on a Leica Autostainer in Xylenes (1X 5 min, 2X 2 min), and rehydrated through Ethanols (2X 100% for 5 min each, 95% 2X 2 min each, 80%, 70%) to running tap water. - Sections were heated to 98°C for 20 min in 10 mM Sodium Citrate buffer pH 6.0 for antigen retrieval, then moved to RT in the same buffer for 20 min.
    • Sections were rinsed in de-ionized water for 5 min at RT.
    • Sections were blocked in Hydrogen Peroxide (Fisher, H325-500) for 30 min at RT.
    • Sections were rinsed in Tris Buffered Saline with 0.5% Tween-20 (TBST) 2 times for 5 min at RT.
    • Sections were blocked in 5% Normal Goat Serum for 2 h at RT.
    • Sections were incubated with primary antibody diluted 1:250 in TBST overnight at 4°C.
    • Sections were rinsed in TBST for 5 min 2X at RT.
    • Sections were incubated with Envision Plus anti-Rabbit-Horse Radish Peroxidase conjugated Polymer for 2 h at RT.
    • Sections were rinsed in TBST for 5 min 2X at RT.
    • Sections were incubated with DAB chromogenic substrate (DAKO, K348) for 10 min at RT.
    • Sections were washed x 1 in Distilled Water.
    • Sections were counterstained with 1:9 dilution of Harris Hematoxylin (Fisher, SH30-500D) for 2 min.
    • Sections were washed x 1 in Distilled Water.
    • Sections were blued in Ammonium Hydroxide for 1 min.
    • Sections were washed x 1 in Distilled Water.
    • Sections were dehydrated through graded alcohols, mounted in Acrymount and photographed on an Olympus DX61 microscope with DP70 camera using DP Controller and DP Manager Software.
    Anmerkungen
    • The company recommends between 1:100 – 1:500 dilution factor; in our hands, 1:250 was overstained and we would recommend using a lower concentration of the antibody.
    • The “normal” brain section we used proved to be from an individual who had morphological features of Parkinson’s Disease. The area shown in Figure 2 is from the morphologically normal part of the brain. The area with Parkinson’s morphology reacted strongly with the anti-wnt2 antibody (Figure 5).
  • Format
    Liquid
    Konzentration
    1 μg/μL
    Buffer
    0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
    Konservierungsmittel
    ProClin
    Vorsichtsmaßnahmen
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
    Haltbarkeit
    12 months
  • Król, Mucha, Majchrzak, Homa, Bulkowska, Majewska, Gajewska, Pietrzak, Perszko, Romanowska, Paw?owski, Manuali, Hellmen, Motyl: "Macrophages mediate a switch between canonical and non-canonical Wnt pathways in canine mammary tumors." in: PLoS ONE, Vol. 9, Issue 1, pp. e83995, (2014) (PubMed).

  • Target
    WNT2 (Wingless-Type MMTV Integration Site Family Member 2 (WNT2))
    Andere Bezeichnung
    WNT2 (WNT2 Produkte)
    Synonyme
    CG1916 antikoerper, D-wnt-2 antikoerper, DWnt-2 antikoerper, DWnt2 antikoerper, Dm DWnt2 antikoerper, Dm-2 antikoerper, Dmel\\CG1916 antikoerper, Dwnt-2 antikoerper, Wnt antikoerper, Wnt-2 antikoerper, dWnt2 antikoerper, wnt2 antikoerper, WNT2 antikoerper, irp antikoerper, Xwnt2 antikoerper, wnt-2 antikoerper, Xwnt-2 antikoerper, int1l1 antikoerper, 2610510E18Rik antikoerper, Int1l1 antikoerper, Irp antikoerper, Mirp antikoerper, Wnt2a antikoerper, INT1L1 antikoerper, IRP antikoerper, ZfWnt2 antikoerper, Wnt oncogene analog 2 antikoerper, Wnt family member 2 antikoerper, wingless-type MMTV integration site family member 2 antikoerper, wingless-type MMTV integration site family, member 2 antikoerper, Wnt2 antikoerper, wnt2 antikoerper, WNT2 antikoerper
    Hintergrund

    Synonyms: IRP, INT1L1, Protein Wnt-2, Int-1-like protein 1, Int-1-related protein, WNT2

    Background: Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters.

    Gen-ID
    7472
    UniProt
    P09544
    Pathways
    WNT Signalweg
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