RPA2 Antikörper

Produktnummer ABIN8124318

Der Kaninchen Monoklonal anti-RPA2 Antikörper wird verwendet zum Nachweis von RPA2 in Proben von Human, Maus und Ratte. Er wurde validiert für WB, IHC, ELISA, IF und IP.

Kurzübersicht für RPA2 Antikörper (ABIN8124318)

Target: RPA2 (Replication Protein A2, 32kDa (RPA2))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser RPA2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP)

Klon: AB1892

Produktdetails anti-RPA2 Antikörper

Verwendungszweck: RPA32/RPA2 Rabbit mAb

Spezifität: This antibody detects endogenous levels of RPA32/RPA2 protein.

Aufreinigung: The antibody was purified by protein A affinity chromatography.

Immunogen: Recombinant protein (or fragment).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB, 1:1000-1:5000 | IHC, 1:1000-1:4000 | IF, 1:200-1:1000 | IP, 0. 5 μg-4 μg antibody for 200 μg-400 μg extracts of whole cells. | ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The antibody is provided in liquid form in phosphate - buffered saline with 50 % glycerol, 0.05 % BSA, and 0.05 % Proclin 300.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid freeze / thaw cycles

Lagerung: -20 °C

Informationen zur Lagerung: Store at-20°C. Avoid freeze / thaw cycles.

Details zu RPA2

Target: RPA2 (Replication Protein A2, 32kDa (RPA2))

Andere Bezeichnung: RPA32/RPA2

Hintergrund:

Synonyms: RPA2, REPA2, RPA32, RPA34, Replication protein A 32 kDa subunit, RP-A p32, Replication factor A protein 2, RF-A protein 2, Replication protein A 34 kDa subunit, RP-A p34

Background: function:Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions. , PTM:Phosphorylated in a cell-cycle-dependent manner (from the S phase until mitosis) . Phosphorylated by ATR upon DNA damage, which promotes its translocation to nuclear foci. Can be phosphorylated in vitro by PRKDC/DNA-PK in the presence of Ku and DNA, and by CDC2. , subcellular location:Also present in PML nuclear bodies. Redistributes to discrete nuclear foci upon DNA damage. , subunit:Heterotrimer of 70, 32 and 14 kDa chains. The DNA-binding activity may reside exclusively on the 70 kDa subunit. Binds to SERTAD3/RBT1. Interacts with TIPIN. ,

Gene Name: RPA2

Protein Name: RPA32/RPA2

Gen-ID: 6118

UniProt: P15927

Pathways: Telomere Maintenance, DNA Reparatur, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA