MASP1 Antikörper (Cleaved-Ile449, Light Chain)

Produktnummer ABIN8122756

Der Kaninchen Polyklonal anti-MASP1 Antikörper (ABIN8122756) detektiert spezifisch MASP1 in WB und ELISA. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus und Ratte.

Kurzübersicht für MASP1 Antikörper (Cleaved-Ile449, Light Chain) (ABIN8122756)

Target: MASP1 (Mannan-Binding Lectin Serine Peptidase 1 (MASP1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MASP1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-MASP1 Antikörper

Bindungsspezifität: Cleaved-Ile449, Light Chain

Verwendungszweck: MASP1 (light chain, Cleaved-Ile449) Antibody

Spezifität: This antibody detects endogenous levels of Human MASP1 (light chain, Cleaved-Ile449, protein was cleaved amino acid sequence between 448-449 )

Aufreinigung: The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Immunogen: Synthesized peptide derived from human MASP1 (light chain, Cleaved-Ile449)

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:1000-2000 ELISA 1:5000-20000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C/1 year

Haltbarkeit: 12 months

Details zu MASP1

Target: MASP1 (Mannan-Binding Lectin Serine Peptidase 1 (MASP1))

Andere Bezeichnung: MASP1

Hintergrund:

Synonyms: Mannan-binding lectin serine protease 1 (EC 3.4.21.-, Complement factor MASP-3, Complement-activating component of Ra-reactive factor, Mannose-binding lectin-associated serine protease 1, MASP-1, Mannose-binding protein-associated serine protease, Ra-reactive factor serine protease p100, RaRF, Serine protease 5) [Cleaved into: Mannan-binding lectin serine protease 1 heavy chain, Mannan-binding lectin serine protease 1 light chain]

Background: enzyme regulation:Inhibited by SERPING1 and A2M.,function:Functions in the lectin pathway of complement, which performs a key role in innate immunity by recognizing pathogens through patterns of sugar moieties and neutralizing them. The lectin pathway is triggered upon binding of mannan-binding lectin (MBL) and ficolins to sugar moieties which leads to activation of the associated proteases MASP1 and MASP2. Functions as an endopeptidase and may activate MASP2 or C2 or directly activate C3 the key component of complement reaction. Isoform 2 may have an inhibitory effect on the activation of the lectin pathway of complement or may cleave IGFBP5.,PTM:Autoproteolytic processing of the proenzyme produces the active enzyme composed on the heavy and the light chain held together by a disulfide bond. Isoform 1 but not isoform 2 is activated through autoproteolytic processing.,PTM:N-glycosylated. Some N-linked glycan are of the complex-type.,PTM:The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.,similarity:Belongs to the peptidase S1 family.,similarity:Contains 1 EGF-like domain.,similarity:Contains 1 peptidase S1 domain.,similarity:Contains 2 CUB domains.,similarity:Contains 2 Sushi (CCP/SCR) domains.,subunit:Homodimer. Interacts with the oligomeric lectins MBL2, FCN2 and FCN3, triggers the lectin pathway of complement through activation of C3. Interacts with SERPING1.,tissue specificity:Protein of the plasma which is primarily expressed by liver.,

Gene Name: MASP1 CRARF CRARF1 PRSS5

Protein Name: MASP1 (light chain, Cleaved-Ile449)

Gen-ID: 5648

UniProt: P48740

Pathways: Komplementsystem