HIST1H3A Antikörper (AA 131-230) (HRP)

Produktnummer ABIN759515

Der Kaninchen Polyklonal Anti-HIST1H3A-Antikörper wurde für ELISA, WB, IHC (p) und IHC (fro) validiert. Er ist geeignet, HIST1H3A in Proben von Human, Maus, Ratte und Schaf zu detektieren.

Kurzübersicht für HIST1H3A Antikörper (AA 131-230) (HRP) (ABIN759515)

Target: HIST1H3A (Histone Cluster 1, H3a (HIST1H3A))

Reaktivität: Human, Maus, Ratte, Schaf

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser HIST1H3A Antikörper ist konjugiert mit HRP

Applikation: ELISA, Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-HIST1H3A Antikörper (HRP)

Bindungsspezifität: AA 131-230

Kreuzreaktivität: Human, Maus, Ratte, Schaf

Homologie: Dog,Cow,Pig,Horse

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human HIF3 alpha

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handhabung: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu HIST1H3A

Target: HIST1H3A (Histone Cluster 1, H3a (HIST1H3A))

Andere Bezeichnung: H3 alpha

Hintergrund:

Synonyms: IPAS, MOP7, PASD7, HIF-3A, bHLHe17, Hypoxia-inducible factor 3-alpha, HIF-3-alpha, HIF3-alpha, Basic-helix-loop-helix-PAS protein MOP7, Class E basic helix-loop-helix protein 17, HIF3-alpha-1, Inhibitory PAS domain protein, Member of PAS protein 7, PAS domain-containing protein 7, HIF3A

Background: Involved in adaptive response to hypoxia. Suppresses hypoxia-inducible expression of HIF1A and EPAS1. Binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters. The complex HIF3A-ARNT activates the transcription of reporter genes driven by HRE. Isoform 4 has a dominant-negative function of inactivating HIF1A-mediated transcription. Isoform 4 attenuates the binding of HIF1A to hypoxia-responsive elements (HRE), thus inhibiting HRE-driven transcription. Hypoxia induces down-regulation of isoform 4, leading to activation of HIF1A in hypoxia. Conversely, upon restoring normoxia, the expression of isoform 4 increases and thereby secure an inhibition of HIF1A activity. Isoform 4 may be a negative regulator of hypoxia-inducible gene expression in the kidney and may be involved in renal tumorigenesis. Functions as an inhibitor of angiogenesis in the cornea (By similarity).

Gen-ID: 64344

UniProt: Q9Y2N7