Rekombinanter SPARC Antikörper (C-Term)

Produktnummer ABIN8093460

Der Maus Monoklonal anti-SPARC Antikörper (Klon r15G12) (ABIN8093460) detektiert spezifisch SPARC in WB und IHC. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für Rekombinanter SPARC Antikörper (C-Term) (ABIN8093460)

Target: SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser SPARC Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Güteklasse: Carrier-free

Klon: R15G12

Produktdetails anti-SPARC Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: SPARC Antibody / Osteonectin (azide and preservative free)

Immunogen: Prokaryotic recombinant protein corresponding to a portion of the C-terminus of the human Osteonectin molecule was used as the immunogen for the Basement Membrane Protein 40/SPARC antibody.

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise: 1. Optimal dilution of the Basement Membrane Protein 40/SPARC antibody should be determined by the researcher.

2. This Basement Membrane Protein 40 antibody is recombinantly produced by expression in CHO cells.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Basement Membrane Protein 40/SPARC antibody with sodium azide store at 2 to 8oC, antibody without sodium azide store at -20 to -80oC.

Details zu SPARC

Target: SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))

Andere Bezeichnung: SPARC

Hintergrund: Basement Membrane Protein 40 antibody, also known as SPARC antibody, recognizes Secreted protein acidic and rich in cysteine, a secreted matricellular glycoprotein that regulates extracellular matrix organization and cell-matrix communication. The human SPARC gene is located on chromosome 5q33.1 and encodes a protein widely referred to as Osteonectin and BM-40 in the literature. SPARC is secreted into the extracellular space and associates with basement membranes and stromal matrices, where it modulates cellular adhesion and matrix assembly rather than serving as a structural scaffold.

Secreted protein acidic and rich in cysteine plays a central role in collagen binding, fibrillogenesis, and regulation of cell migration and proliferation. It is highly expressed in connective tissues, bone, endothelial cells, and activated fibroblasts. In skeletal biology, Osteonectin is abundant in osteoblasts and mineralizing bone matrix, contributing to collagen organization and tissue mineralization. A Basement Membrane Protein 40 antibody is commonly used in research examining extracellular matrix remodeling, tumor microenvironment dynamics, angiogenesis, and fibrotic disease because SPARC influences stromal activation and matrix composition.

Structurally, SPARC contains an acidic N-terminal region, a follistatin-like domain, and a C-terminal extracellular calcium-binding domain with EF-hand motifs. These domains enable calcium binding and mediate interactions with collagens, albumin, and growth factors. Through these interactions, Secreted protein acidic and rich in cysteine participates in signaling pathways such as TGF-beta signaling and integrin-mediated adhesion cascades, supporting tissue remodeling and vascular responses.

In cancer biology, SPARC expression is frequently detected in desmoplastic stroma associated with breast, pancreatic, colorectal, and ovarian carcinomas. Expression is often observed in tumor-associated fibroblasts and matrix-rich regions, where SPARC contributes to extracellular matrix reorganization. Depending on tumor context, SPARC may influence invasion, stromal remodeling, and growth factor responsiveness. Outside oncology, SPARC is upregulated during wound healing and in fibrotic conditions affecting liver, lung, and kidney, reflecting its broader role in tissue repair and matrix turnover. During embryonic development, SPARC expression is present in tissues undergoing active morphogenesis and vascular formation.

Clone r15G12 is a recombinant monoclonal antibody developed to recognize SPARC for research applications. A Basement Membrane Protein 40 antibody supports investigation of extracellular matrix biology, stromal cell function, and connective tissue remodeling. This antibody targets SPARC in research settings and is suitable for studies of fibrosis, angiogenesis, skeletal biology, and tumor-associated stromal responses.

UniProt: P09486

Pathways: Autophagie