Rekombinanter MCM4 Antikörper (AA 600-800)

Produktnummer ABIN8093330

Der Kaninchen Monoklonal anti-MCM4 Antikörper wird verwendet zum Nachweis von MCM4 in Proben von Human. Er wurde validiert für WB und IHC.

Kurzübersicht für Rekombinanter MCM4 Antikörper (AA 600-800) (ABIN8093330)

Target: MCM4 (Minichromosome Maintenance Deficient 4 (MCM4))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser MCM4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Güteklasse: Carrier-free

Klon: MCM4-13209R

Produktdetails anti-MCM4 Antikörper

Bindungsspezifität: AA 600-800

Verwendungszweck: MCM4 Antibody / DNA replication licensing factor MCM4 (azide and preservative free)

Immunogen: A recombinant fragment (around amino acids 600-800) of human MCM4 protein (exact sequence is proprietary) was used as the immunogen for the recombinant MCM4/Cell proliferation marker MCM4 antibody.

Isotyp: IgG, kappa

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the recombinant MCM4/Cell proliferation marker MCM4 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: MCM4/Cell proliferation marker MCM4 antibody with sodium azide - store at 2 to 8oC, antibody without sodium azide - store at -20 to -80oC.

Details zu MCM4

Target: MCM4 (Minichromosome Maintenance Deficient 4 (MCM4))

Andere Bezeichnung: MCM4

Hintergrund: MCM4/Cell proliferation marker MCM4 Antibody recognizes Minichromosome maintenance complex component 4, also known as DNA replication licensing factor 4 and commonly described as a cell proliferation marker MCM4 in pathology literature. The MCM4 gene encodes a nuclear protein that forms part of the heterohexameric MCM2-7 complex, the core replicative helicase responsible for unwinding DNA during S phase. Because of its central role in replication licensing and DNA synthesis, MCM4 antibody is widely used as a sensitive indicator of proliferating cells in normal and neoplastic tissues.

MCM4 is expressed in the nuclei of cells that are actively cycling, including those in G1, S, G2, and M phases, but is absent in quiescent or terminally differentiated cells. This expression pattern makes cell proliferation marker MCM4 antibody particularly useful for identifying growth fractions in tumors. Compared with traditional proliferation markers, MCM4 often highlights a broader population of cycling cells, reflecting its role in replication origin licensing before DNA synthesis begins.

At the molecular level, MCM4 interacts with MCM2, MCM3, MCM5, MCM6, and MCM7 to assemble the functional helicase complex. Activation of this complex requires sequential phosphorylation events mediated by cyclin-dependent kinases and DDK, enabling origin firing and fork progression. Dysregulation of MCM4 expression has been associated with genomic instability and tumor progression in several malignancies, including colorectal carcinoma, breast carcinoma, lung carcinoma, and gynecologic cancers. Elevated nuclear labeling with MCM4 antibody frequently correlates with high proliferative index and aggressive clinicopathologic features.

In immunohistochemistry, MCM4 antibody produces distinct nuclear staining in proliferating epithelial, lymphoid, and mesenchymal cells, while non-dividing stromal or differentiated cells typically remain negative. This selective nuclear localization aligns with its biological function in DNA replication. Clone MCM4/13209R is designed to detect endogenous MCM4 protein in research applications and provides clear nuclear labeling suitable for evaluating proliferative activity in formalin-fixed, paraffin-embedded tissue sections.

By targeting a key replication licensing factor, MCM4 antibody serves as a valuable tool for studying cell cycle regulation, oncogenesis, and tissue growth dynamics. Its established role as a cell proliferation marker MCM4 makes it particularly relevant for cancer research and for comparative analyses of proliferative indices across different tissue types and tumor grades.

UniProt: P33991

Pathways: DNA Reparatur, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA