Rekombinanter LIPE Antikörper (pSer853)

Produktnummer ABIN8093300

Der Kaninchen Monoklonal anti-LIPE Antikörper wird verwendet zum Nachweis von LIPE in Proben von Human, Maus und Ratte. Er wurde validiert für WB und IHC.

Kurzübersicht für Rekombinanter LIPE Antikörper (pSer853) (ABIN8093300)

Target: LIPE (Lipase, Hormone-Sensitive (LIPE))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser LIPE Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Klon: FDO-12

Produktdetails anti-LIPE Antikörper

Bindungsspezifität: pSer853

Verwendungszweck: Phospho-LIPE (pSer853) Antibody / Hormone-sensitive lipase / HSL

Aufreinigung: Affinity chromatography

Immunogen: A synthesized peptide derived from human Phospho-Hormone-sensitive lipase (pS853) was used as the immunogen for the Phospho-LIPE (pSer853) antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the Phospho-LIPE (pSer853) antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store the Phospho-LIPE antibody at -20oC.

Details zu LIPE

Target: LIPE (Lipase, Hormone-Sensitive (LIPE))

Andere Bezeichnung: LIPE

Hintergrund: Phospho-LIPE (pSer853) antibody targets the phosphorylated form of Hormone-sensitive lipase (LIPE), also known as HSL, at serine 853, a well-characterized regulatory phosphorylation site associated with activation of lipolytic function. LIPE is a cytoplasmic neutral lipase that plays a central role in lipid mobilization by hydrolyzing triglycerides, diacylglycerols, and cholesteryl esters stored within lipid droplets. In metabolic cells, LIPE localizes primarily to the cytoplasm and lipid droplet surface, with phosphorylation-dependent activation in response to hormonal and signaling cues. Detection of phospho-LIPE provides insight into the activated, signal-responsive pool of HSL rather than total protein abundance.

Functionally, phosphorylation of LIPE at serine 853 is linked to kinase-driven signaling pathways that regulate lipolysis during periods of increased energy demand. This modification promotes enzymatic activation and facilitates interaction with lipid droplets, enabling efficient release of free fatty acids. Hormonal stimulation, such as catecholamine signaling, enhances LIPE phosphorylation, while opposing pathways suppress phosphorylation and favor lipid storage. A Phospho-LIPE (pSer853) antibody supports studies examining dynamic regulation of lipolysis and metabolic signaling pathways.

Phosphorylated LIPE plays an important role in adipocytes, skeletal muscle, and steroidogenic tissues, where controlled lipid mobilization is essential for energy production and biosynthetic processes. In adipose tissue, LIPE phosphorylation coordinates lipid breakdown during fasting or stress, while in muscle it contributes to fuel availability during energy demand. Monitoring phospho-LIPE levels enables assessment of pathway activation states rather than total enzyme expression, providing a functional readout of lipid metabolic regulation.

From a biological and disease-relevance perspective, dysregulation of LIPE phosphorylation is associated with altered lipid handling, insulin resistance, and metabolic disease. Aberrant signaling that affects LIPE activation can lead to impaired fatty acid release or excessive lipid accumulation. Phospho-LIPE therefore represents a valuable marker for studying signaling pathways that control lipid metabolism, metabolic flexibility, and hormone-responsive energy balance.

At the molecular level, LIPE is encoded by the LIPE gene and produces a cytoplasmic enzyme that migrates at approximately 80-90 kDa on SDS-PAGE under reducing conditions. Phosphorylation-dependent regulation of LIPE occurs in response to cellular metabolic state and upstream kinase activity. A Phospho-LIPE (pSer853) antibody supports research applications focused on lipid signaling, metabolic regulation, and post-translational control of hormone-sensitive lipase, with NSJ Bioreagents providing reagents intended for research use.

UniProt: Q05469

Pathways: AMPK Signaling, Monocarboxylic Acid Catabolic Process, Lipid Metabolism