Indian Hedgehog Antikörper (AA 227-360)

Produktnummer ABIN8093262

Der Kaninchen Polyklonal anti-Indian Hedgehog Antikörper (ABIN8093262) detektiert spezifisch Indian Hedgehog in WB, ELISA, IHC, FACS und IF. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus und Ratte.

Kurzübersicht für Indian Hedgehog Antikörper (AA 227-360) (ABIN8093262)

Target: Indian Hedgehog (IHH)

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Indian Hedgehog Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Produktdetails anti-Indian Hedgehog Antikörper

Bindungsspezifität: AA 227-360

Verwendungszweck: IHH Antibody / Indian hedgehog protein

Aufreinigung: Immunogen affinity purified

Immunogen: E.coli-derived human Indian hedgehog protein recombinant protein (amino acids D227-A360) was used as the immunogen for the IHH antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the IHH antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the IHH antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu Indian Hedgehog

Target: Indian Hedgehog (IHH)

Andere Bezeichnung: IHH

Hintergrund: IHH antibody targets Indian hedgehog protein, encoded by the IHH gene. Indian hedgehog protein is a secreted signaling molecule belonging to the hedgehog family and plays a central role in embryonic development and tissue patterning. IHH is synthesized as a precursor protein that undergoes autocatalytic cleavage to generate an active N-terminal signaling fragment responsible for pathway activation.

Functionally, Indian hedgehog protein regulates cell proliferation and differentiation through activation of the hedgehog signaling pathway. By interacting with Patched receptors and modulating downstream Gli transcription factors, IHH coordinates communication between neighboring cell populations. An IHH antibody supports studies focused on morphogen signaling and developmental regulation.

IHH expression is especially prominent in skeletal tissues, where it is produced by prehypertrophic chondrocytes during bone development. In this context, Indian hedgehog protein governs the balance between cartilage growth and maturation by coordinating feedback signaling with surrounding perichondrial cells. This spatially restricted expression highlights its importance in endochondral ossification.

From a disease-relevance perspective, dysregulation of Indian hedgehog protein signaling has been linked to skeletal abnormalities and growth plate disorders. Aberrant IHH pathway activity has also been examined in cancer and fibrotic disease, where reactivation of developmental signaling contributes to pathological tissue remodeling.

At the molecular level, Indian hedgehog protein undergoes lipid modification that influences secretion, signaling range, and gradient formation. These processing events can affect its apparent behavior in biochemical assays without altering the primary sequence. IHH antibody reagents support research applications focused on developmental biology and hedgehog pathway signaling, with NSJ Bioreagents providing reagents intended for research use.

UniProt: Q14623

Pathways: Hedgehog Signalweg