RHOA Antikörper (pSer188)

Produktnummer ABIN758242

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch RHOA in WB, ELISA, IHC (p), FACS, IF (cc), IF (p) und IHC (fro). Er zeigt eine Reaktivität gegenüber Human, Maus und Ratte und wurde in 2+ Publikationen erwähnt.

Kurzübersicht für RHOA Antikörper (pSer188) (ABIN758242)

Target: RHOA (Ras Homolog Gene Family, Member A (RHOA))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RHOA Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-RHOA Antikörper

Bindungsspezifität: pSer188

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Dog,Cow,Sheep,Chicken

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human RhoA around the phosphorylation site of Ser188

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit: 12 months

Referenzen für anti-RHOA Antikörper (ABIN758242)

Gu, Feng, Yu, Yuan, Yin, Ding, Zhao, Xu, Xu, Che: "Fasudil attenuates soluble fms-like tyrosine kinase-1 (sFlt-1)-induced hypertension in pregnant mice through RhoA/ROCK pathway." in: Oncotarget, Vol. 8, Issue 61, pp. 104104-104112, (2017) (PubMed).

Zhang, He, Lu, Bian, Yang, Fu, Wu, Li: "HSF1 Relieves Amyloid-?-Induced Cardiomyocytes Apoptosis." in: Cell biochemistry and biophysics, (2015) (PubMed).

Details zu RHOA

Target: RHOA (Ras Homolog Gene Family, Member A (RHOA))

Andere Bezeichnung: RhoA

Hintergrund:

Synonyms: RHOAphospho S188, RhoA protein, Aplysia ras related homolog 12, ARH 12, ARH12, ARHA, H 12, Oncogene RHO H12, Ras homolog gene family member A, RHO 12, Rho A, RHO H12, RHO12, RHOH 12, RHOH12, Small GTP binding protein Rho A, Small GTP binding protein RhoA, Transforming protein Rho A, Transforming protein RhoA.

Background: This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. The protein encoded by this gene is prenylated at its C-terminus, and localizes to the cytoplasm and plasma membrane. It is thought to be important in cell locomotion. Overexpression of this gene is associated with tumor cell proliferation and metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Gen-ID: 387

Pathways: Microtubule Dynamics, WNT Signalweg, Neurotrophin Signalübertragung, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Actin Filament Polymerization, Cell-Cell Junction Organization, Positive Regulation of Endopeptidase Activity, Signaling Events mediated by VEGFR1 and VEGFR2, Thromboxane A2 Receptor Signaling, SARS-CoV-2 Protein Interaktom