TUBB Antikörper

Produktnummer ABIN8071385

Dieser Maus Monoklonal Antikörper detektiert spezifisch TUBB in WB, IHC, ICC und IP. Es zeigt Reaktivität gegenüber Proben von Human, Maus und Paramecium.

Kurzübersicht für TUBB Antikörper (ABIN8071385)

Target: TUBB (Tubulin, beta (TUBB))

Reaktivität: Human, Maus, Paramecium

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser TUBB Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Klon: AXO45

Produktdetails anti-TUBB Antikörper

Verwendungszweck: anti-beta-Tubulin, mAb (AXO45)

Spezifität: Detects almost all isotypes of beta-tubulin specifically in cells and tissues of mouse, human and ciliates.

Produktmerkmale: Monoclonal Antibody. Detects almost all isotypes of β-tubulin specifically in cells and tissues of mouse, human and ciliates. Applications: ICC, IHC, IP and WB. Clone: AXO45. Isotype: Mouse IgG1. The β-tubulin monoclonal antibody (AXO45) was developed by isolating the cilia from unicellular multiciliated Paramecium and purifying the core microtubule-based structures of the cilia, the axonemes. These axonemes were electrophoresed on a SDS-PAGE and the tubulin band was used as an antigen to develop monoclonal antibodies targeting both α- and β-tubulin (A.M. Callen, et al., 1994). The antibody AXO45 recognizes the C-terminal region between residues 257 and 442 of β-tubulin. Although the antibody was raised against the β-tubulin found in cilia, it is able to recognize β-tubulin of all microtubule networks, indicating that it can potentially recognize different isotypes of β-tubulin found in mammals.

Reinheit: >95 % (SDS-PAGE)

Immunogen: Axonemal tubulin from the ciliate Paramecium electroeluted from the 55 kDa band of the SDS-PAGE gel.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: In PBS containing 0.02 % Proclin 300.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: After opening, prepare aliquots and store at -20 °C.Avoid freeze/thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

+4°C

-20°C

Stable for at least 1 month after receipt when stored at +4°C. Stable for at least 1 year after receipt when stored at -20°C.

Haltbarkeit: 12 months

Details zu TUBB

Target: TUBB (Tubulin, beta (TUBB))

Andere Bezeichnung: beta-Tubulin

Hintergrund:

Alternate Names/Synonyms: Tubulin beta Chain, Tubulin beta-5 Chain

Product Description: The beta-tubulin monoclonal antibody (AXO45) was developed by isolating the cilia from unicellular multiciliated Paramecium and purifying the core microtubule-based structures of the cilia, the axonemes. These axonemes were electrophoresed on a SDS-PAGE and the tubulin band was used as an antigen to develop monoclonal antibodies targeting both alpha- and beta-tubulin (A.M. Callen, et al., 1994). The antibody AXO45 recognizes the C-terminal region between residues 257 and 442 of beta-tubulin. Although the antibody was raised against the beta-tubulin found in cilia, it is able to recognize beta-tubulin of all microtubule networks, indicating that it can potentially recognize different isotypes of beta-tubulin found in mammals.

Pathways: Microtubule Dynamics, M Phase