AKT 1/2/3 Antikörper (pTyr312, pTyr315, pTyr316) (PE-Cy5)

Produktnummer ABIN756229

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch AKT 1/2/3 in WB und FACS. Es zeigt Reaktivität gegenüber Proben von Human, Maus, Ratte, Rind (Kuh), Huhn, Schwein und Hund.

Kurzübersicht für AKT 1/2/3 Antikörper (pTyr312, pTyr315, pTyr316) (PE-Cy5) (ABIN756229)

Target: AKT 1/2/3

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Huhn, Schwein, Hund

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser AKT 1/2/3 Antikörper ist konjugiert mit PE-Cy5

Applikation: Western Blotting (WB), Flow Cytometry (FACS)

Produktdetails anti-AKT 1/2/3 Antikörper (PE-Cy5)

Bindungsspezifität: pTyr312, pTyr315, pTyr316

Spezifität: This antibody will detect AKT 1 when phosphorylated at Tyr315 in Human, Mouse, Rat or Bovine, AKT2 when phosphorylated at Tyr316 in Human, Mouse, Rat, or Bovine, and AKT3 when phosphorylated at Tyr312 in Human, Mouse, Rat, or Bovine.

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Dog,Cow,Sheep,Pig,Chicken,Rabbit

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human AKT1 around the phosphorylation site of Tyr315

Isotyp: IgG

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu AKT 1/2/3

Target: AKT 1/2/3

Andere Bezeichnung: AKT1+2+3

Hintergrund:

Synonyms: AKT1/AKT2/AKT3 Tyr315+Tyr316+Tyr312, AKT1/AKT2/AKT3Tyr315/316/312, AKT1/2/3 phospho Y315 + Y316 + Y312, AKT 1, AKT, AKT1, AKT-1, AKT1_HUMAN, AKT 2, AKT2, AKT-2, AKT2_HUMAN, AKT 3, AKT3, AKT-3, AKT3_HUMAN, C AKT, cAKT, MGC9965, MGC99656, Oncogene AKT1, PKB, PKB alpha, PKB-ALPHA, PKB beta, PKB gamma, PRKBA, Protein Kinase B Alpha, Protein kinase B, Proto-oncogene c-Akt, RAC Alpha, RAC alpha serine/threonine protein kinase, RAC, RAC PK Alpha, Rac protein kinase alpha, RAC Serine/Threonine Protein Kinase, RAC-alpha serine/threonine-protein kinase, RAC-PK-alpha, v akt murine thymoma viral oncogene homolog 1, vAKT Murine Thymoma Viral Oncogene Homolog 1.

Background: AKT, also known as protein kinase B (PKB), is a 57 kDa serine/threonine protein kinase. There are three mammalian isoforms of Akt: AKT1 (PKB alpha), AKT2 (PKB beta) and AKT3 (PKB gamma) with AKT2 and AKT3 being approximately 82 % identical with the AKT1 isoform. Each isoform has a pleckstrin homology (PH)domain, a kinase domain and a carboxy terminal regulatory domain. AKT was originally cloned from the retrovirus AKT8, and is a key regulator of many signal transduction pathways. Its tight control over cell proliferation and cell viability are manifold, overexpression or inappropriate activation of AKT has been seen in many types of cancer. AKT mediates many of the downstream events of phosphatidylinositol 3 kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI3 kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus.

Molekulargewicht: 56kDa

Gen-ID: 207, 208, 10000