C1S Antikörper

Produktnummer ABIN8065539

Der Maus Monoklonal anti-C1S Antikörper wird verwendet zum Nachweis von C1S in Proben von Human. Er wurde validiert für ELISA und IF.

Kurzübersicht für C1S Antikörper (ABIN8065539)

Target: C1S (Complement Component 1, S Subcomponent (C1S))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser C1S Antikörper ist unkonjugiert

Applikation: ELISA, Immunofluorescence (IF)

Güteklasse: Carrier-free

Klon: 49

Produktdetails anti-C1S Antikörper

Verwendungszweck: Anti-Pro/Actived Complement C1s Antibody

Spezifität: ABS 002-49 is specific for C1s proenzyme and activated free C1s and C1s in complex with C1 inhibitor. Epitope differs from that of ABS 002-09.

Homologie: Human

Aufreinigung: Protein A or Protein G purified

Immunogen: Human plasma C1s Proenzyme

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: 2-8°C without exposure to light

Haltbarkeit: 24 months

Details zu C1S

Target: C1S (Complement Component 1, S Subcomponent (C1S))

Andere Bezeichnung: Pro/Actived Complement C1s

Hintergrund: C1S, EDSPD2, complement C1s subcomponent, C1 esterase, basic proline-rich peptide IB-1, complement component 1 subcomponent s, complement component 1, s subcomponent,Component of the complement C1 complex, a multiprotein complex that initiates the classical pathway of the complement system, a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system (PubMed:11445589, PubMed:16169853, PubMed:417728, PubMed:467643, PubMed:6271784, PubMed:6282646, PubMed:6319179, PubMed:70787, PubMed:9422791). C1S is activated following association of the C1 complex with immunoglobulins (IgG or IgM) complexed with antigens to form antigen-antibody complexes on the surface of pathogens (PubMed:34155115). C1S is cleaved and activated by C1R to generate C1s subcomponent heavy and light chains (PubMed:11445589, PubMed:6271784). C1s subcomponent light chain then cleaves and activates C2 and C4, the next components of the classical complement pathway (PubMed:16169853, PubMed:467643, PubMed:6282646, PubMed:6319179, PubMed:6906228, PubMed:70787, PubMed:9422791). {ECO:0000269|PubMed:11445589, ECO:0000269|PubMed:16169853, ECO:0000269|PubMed:34155115, ECO:0000269|PubMed:417728, ECO:0000269|PubMed:467643, ECO:0000269|PubMed:6271784, ECO:0000269|PubMed:6282646, ECO:0000269|PubMed:6319179, ECO:0000269|PubMed:6906228, ECO:0000269|PubMed:70787, ECO:0000269|PubMed:9422791}., [Complement C1s subcomponent light chain]: Serine protease component of the complement C1 complex, which catalyzes cleavage and activation of C2 and C4, the next components of the classical complement pathway (PubMed:16169853, PubMed:22949645, PubMed:417728, PubMed:467643, PubMed:6282646, PubMed:6319179, PubMed:70787, PubMed:9422791). Also able to cleave C1 inhibitor (SERPING1) in vitro, additional evidence is however required to confirm this result in vivo (PubMed:16169853). Also cleaves IGFBP5 and thereby inhibits the trophic effects of IGF1 (PubMed:18930415). {ECO:0000269|PubMed:16169853, ECO:0000269|PubMed:18930415, ECO:0000269|PubMed:22949645, ECO:0000269|PubMed:417728, ECO:0000269|PubMed:467643, ECO:0000269|PubMed:6282646, ECO:0000269|PubMed:6319179, ECO:0000269|PubMed:70787, ECO:0000269|PubMed:9422791}.,C1S,C1s proenzyme is a 79.8 kDa glycoprotein produced primarily by hepatocytes but also by other cells. The proenzyme is produced as a single-chain polypeptide of 688 aa. Upon activation the peptide is cleaved between R437 and I438 giving the activated enzyme consisting of two disulfide-linked chains, A chain (52.2 kDa) and B chain (27.7 kDa). The Ca-dependent catalytic subunit of the C1 complex (C1q, C1r, C1s) comprises of two C1s and two C1r molecules and this serine protease tetramer is responsible for the initial steps in the classical complement pathway, cleavage of C4 and C2.

Molekulargewicht: Pro-enzyme C1s (pro-C1s) appears as a single-chain polypeptide around 80 kDa. Upon activation, it is cleaved into two disulfide-linked chains: a ~57 kDa A-chain and and 27 kDa B-chain).

Gen-ID: 716

UniProt: P09871

Pathways: Komplementsystem