POLB Antikörper (AA 122-335)

Produktnummer ABIN8024097

Der Kaninchen Polyklonal anti-POLB Antikörper wird verwendet zum Nachweis von POLB in Proben von Human, Maus und Ratte. Er wurde validiert für WB.

Kurzübersicht für POLB Antikörper (AA 122-335) (ABIN8024097)

Target: POLB (Polymerase (DNA Directed), beta (POLB))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser POLB Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Güteklasse: Carrier-free

Produktdetails anti-POLB Antikörper

Bindungsspezifität: AA 122-335

Verwendungszweck: Anti-DNA Polymerase beta/POLB Antibody

Spezifität: No cross reactivity with other proteins.

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Homologie: Human POLB shares 95.3%,94.9% amino acid (aa) sequence identity with mouse,rat POLB,respectively.

Produktmerkmale: Anti-DNA Polymerase beta/POLB Antibody. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human POLB recombinant protein (Position: L122-E335). Human POLB shares 95.3% and 94.9% amino acid (aa) sequence identity with mouse and rat POLB, respectively.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu POLB

Target: POLB (Polymerase (DNA Directed), beta (POLB))

Andere Bezeichnung: POLB

Hintergrund:

Background: Polymerase (DNA ed), beta, also known as POLB, is an enzyme that, in humans, is encoded by the POLB gene. It is localized on 8p11.2. The protein encoded by this gene is a DNA polymerase involved in base excision and repair, also called gap-filling DNA synthesis. It is found that a truncated POLB is expressed in primary colorectal tumors and inhibits the normal repair function of wildtype POLB. The encoded protein, acting as a monomer, is normally found in the cytoplasm, but it translocates to the nucleus upon DNA damage. Several transcript variants of this gene exist, but the full-length nature of only one has been described to date. Additionally, human POLB forms a complex with and is methylated by PRMT6. In vitro, methylated POLB possesses significantly higher DNA polymerase activity when compared to that of unmodified enzyme. The increase in DNA polymerase activity upon methylation is due to the enhanced DNA binding and processivity of POLB.

Gene Full Name: DNA polymerase beta

Molekulargewicht: 38 kDa

Gen-ID: 5423

UniProt: P06746

Pathways: DNA Reparatur