POLB Antikörper (AA 122-335) (FITC)

Produktnummer ABIN8024092

Der FITC-konjugierte Kaninchen Polyklonal anti-POLB Antikörper (ABIN8024092) detektiert spezifisch POLB in FACS. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus und Ratte.

Kurzübersicht für POLB Antikörper (AA 122-335) (FITC) (ABIN8024092)

Target: POLB (Polymerase (DNA Directed), beta (POLB))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser POLB Antikörper ist konjugiert mit FITC

Applikation: Flow Cytometry (FACS)

Produktdetails anti-POLB Antikörper (FITC)

Bindungsspezifität: AA 122-335

Verwendungszweck: Anti-DNA Polymerase beta/POLB Antibody FITC Conjugated

Spezifität: No cross reactivity with other proteins.

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Homologie: Human POLB shares 95.3%,94.9% amino acid (aa) sequence identity with mouse,rat POLB,respectively.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human POLB recombinant protein (Position: L122-E335). Human POLB shares 95.3% and 94.9% amino acid (aa) sequence identity with mouse and rat POLB, respectively.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Flow Cytometry, Optimal dilutions should be determined by end users.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Haltbarkeit: 12 months

Details zu POLB

Target: POLB (Polymerase (DNA Directed), beta (POLB))

Andere Bezeichnung: POLB

Hintergrund:

Background: Polymerase (DNA ed), beta, also known as POLB, is an enzyme that, in humans, is encoded by the POLB gene. It is localized on 8p11.2. The protein encoded by this gene is a DNA polymerase involved in base excision and repair, also called gap-filling DNA synthesis. It is found that a truncated POLB is expressed in primary colorectal tumors and inhibits the normal repair function of wildtype POLB. The encoded protein, acting as a monomer, is normally found in the cytoplasm, but it translocates to the nucleus upon DNA damage. Several transcript variants of this gene exist, but the full-length nature of only one has been described to date. Additionally, human POLB forms a complex with and is methylated by PRMT6. In vitro, methylated POLB possesses significantly higher DNA polymerase activity when compared to that of unmodified enzyme. The increase in DNA polymerase activity upon methylation is due to the enhanced DNA binding and processivity of POLB.

Gene Full Name: DNA polymerase beta

Gen-ID: 5423

UniProt: P06746

Pathways: DNA Reparatur