Nibrin Antikörper (C-Term)

Produktnummer ABIN8015427

Der Maus Monoklonal anti-Nibrin Antikörper wird verwendet zum Nachweis von Nibrin in Proben von Human, Maus und Ratte. Er wurde validiert für WB, IHC, IF, ICC und FACS.

Kurzübersicht für Nibrin Antikörper (C-Term) (ABIN8015427)

Target: Nibrin (NBN)

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Nibrin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Güteklasse: Carrier-free

Klon: 5D7A1

Produktdetails anti-Nibrin Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: Anti-p95 NBS1 Antibody (monoclonal, 5D7A1)

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Homologie: different from the related mouse sequence by three amino acids,and from the related rat sequence by five amino acids.

Produktmerkmale: Anti-p95 NBS1 Antibody.(monoclonal, 5D7A1)-1. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human p95 NBS1, different from the related mouse sequence by three amino acids, and from the related rat sequence by five amino acids.

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x⁶ cells, Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Details zu Nibrin

Target: Nibrin (NBN)

Andere Bezeichnung: NBN

Hintergrund:

Background: p95 NBS1, also known as NBN or Nibrin, is a protein which in humans is encoded by the NBN gene. Nibrin is a protein associated with the repair of double strand breaks (DSBs) which pose serious damage to a genome. It is a 754 amino acid protein identified as a member of the NBS1/hMre11/RAD50(N/M/R, more commonly referred to asMRN) double strand DNA break repair complex. This complex recognizes DNA damage and rapidly relocates to DSB sites and forms nuclear foci. It also has a role in regulation of N/M/R (MRN) protein complex activity which includes end-processing of both physiological and mutagenic DNA double strand breaks (DSBs).

Gene Full Name: nibrin

Molekulargewicht: 95 kDa

Gen-ID: 4683

UniProt: O60934

Pathways: DNA Reparatur, Production of Molecular Mediator of Immune Response