N4BP1 Antikörper (AA 1-885) (PE)

Produktnummer ABIN8014127

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch N4BP1 in FACS. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für N4BP1 Antikörper (AA 1-885) (PE) (ABIN8014127)

Target: N4BP1 (NEDD4 Binding Protein 1 (N4BP1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser N4BP1 Antikörper ist konjugiert mit PE

Applikation: Flow Cytometry (FACS)

Produktdetails anti-N4BP1 Antikörper (PE)

Bindungsspezifität: AA 1-885

Verwendungszweck: Anti-N4BP1 Antibody PE Conjugated

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human N4BP1 recombinant protein (Position: M1-K885).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Flow Cytometry, Optimal dilutions should be determined by end users.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Haltbarkeit: 12 months

Details zu N4BP1

Target: N4BP1 (NEDD4 Binding Protein 1 (N4BP1))

Andere Bezeichnung: N4BP1

Hintergrund:

Background: Nedd4-binding partner-1 (N4BP1) has been identified as a protein interactor and a substrate of the homologous to E6AP C terminus (HECT) domain-containing E3 ubiquitin-protein ligase (E3), Nedd4. Here, we describe a previously unrecognized functional interaction between N4BP1 and Itch, a Nedd4 structurally related E3, which contains four WW domains, conferring substrate-binding activity. We show that N4BP1 association with the second WW domain (WW2) of Itch interferes with E3 binding to its substrates. In particular, we found that N4BP1 and p73α, a target of Itch-mediated ubiquitin/proteasome proteolysis, share the same binding site. By competing with p73α for binding to the WW2 domain, N4BP1 reduces the ability of Itch to recruit and ubiquitylate p73α and inhibits Itch autoubiquitylation activity both in in vitro and in vivo ubiquitylation assays.

Gene Full Name: NEDD4 binding protein 1

Gen-ID: 9683

UniProt: O75113