MAP3K3 Antikörper (N-Term)

Produktnummer ABIN8011300

Der Kaninchen Polyklonal anti-MAP3K3 Antikörper (ABIN8011300) detektiert spezifisch MAP3K3 in WB. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für MAP3K3 Antikörper (N-Term) (ABIN8011300)

Target: MAP3K3 (Mitogen-Activated Protein Kinase Kinase Kinase 3 (MAP3K3))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MAP3K3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Güteklasse: Carrier-free

Produktdetails anti-MAP3K3 Antikörper

Bindungsspezifität: N-Term

Verwendungszweck: Anti-MEKK3/MAP3K3 Antibody

Spezifität: No cross reactivity with other proteins.

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-MEKK3/MAP3K3 Antibody. Tested in WB applications. This antibody reacts with Human.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human MEKK3.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide, Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu MAP3K3

Target: MAP3K3 (Mitogen-Activated Protein Kinase Kinase Kinase 3 (MAP3K3))

Andere Bezeichnung: MAP3K3

Hintergrund:

Background: MAP3K3 (Mitogen-activated protein kinase kinase kinase 3), also known as MAP/ERK KINASE KINASE 3, MEKK3 or MAPKKK3, is an enzyme that in humans is encoded by the MAP3K3 gene. MAP3K3 is contiguously distal to LYK5 on chromosome 17 (Puffenberger et al., 2007). By yeast 2-hybrid analysis of a mouse T-cell cDNA library, Uhlik et al. (2003) showed that a C-terminal fragment of mouse Osm (CCM2) interacted with Mekk3, which activates p38 in response to sorbitol-induced hyperosmotic conditions. Mekk3 and Osm colocalized in the cytoplasmic compartment of cotransfected cells, and the Mekk3-Osm complex was recruited to Rac1 and cytoskeletal actin-containing membrane ruffles in response to sorbitol treatment. Protein interaction assays showed that Osm interacted ly with the Mekk3 substrate Mkk3 (MAP2K3), with actin, and with both GDP- and GTP-loaded Rac1. Uhlik et al. (2003) concluded that the RAC1-OSM-MEKK3-MKK3 complex is required for regulation of p38 activity in response to osmotic shock.

Gene Full Name: mitogen-activated protein kinase kinase kinase 3

Sequence Similarities: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.

Molekulargewicht: 71 kDa

Gen-ID: 4215

UniProt: Q99759

Pathways: MAPK Signalweg