EIF4E Antikörper (AA 18-217)

Produktnummer ABIN7989055

Der Kaninchen Polyklonal anti-EIF4E Antikörper wird verwendet zum Nachweis von EIF4E in Proben von Human, Ratte und Maus. Er wurde validiert für WB, ELISA, IF, FACS und ICC.

Kurzübersicht für EIF4E Antikörper (AA 18-217) (ABIN7989055)

Target: EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser EIF4E Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Güteklasse: Carrier-free

Produktdetails anti-EIF4E Antikörper

Bindungsspezifität: AA 18-217

Verwendungszweck: Anti-EIF4E Antibody

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-EIF4E Antibody. Tested in WB, ICC, IF, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human EIF4E recombinant protein (Position: E18-V217).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for six months. Avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Details zu EIF4E

Target: EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))

Andere Bezeichnung: EIF4E

Hintergrund:

Background: The protein encoded by this gene is a component of the eukaryotic translation initiation factor 4F complex, which recognizes the 7-methylguanosine cap structure at the 5' end of messenger RNAs. The encoded protein aids in translation initiation by recruiting ribosomes to the 5'-cap structure. Association of this protein with the 4F complex is the rate-limiting step in translation initiation. This gene acts as a proto-oncogene, and its expression and activation is associated with transformation and tumorigenesis. Several pseudogenes of this gene are found on other chromosomes. Alternative splicing results in multiple transcript variants.

Gene Full Name: eukaryotic translation initiation factor 4E

Molekulargewicht: 25 kDa

Gen-ID: 1977

UniProt: P06730

Pathways: BCR Signaling