Dihydrofolate Reductase Antikörper (AA 2-187) (Fluoro488)

Produktnummer ABIN7985248

Der Kaninchen Polyklonal anti-Dihydrofolate Reductase Antikörper wird verwendet zum Nachweis von Dihydrofolate Reductase in Proben von Human, Maus und Ratte. Er wurde validiert für FACS.

Kurzübersicht für Dihydrofolate Reductase Antikörper (AA 2-187) (Fluoro488) (ABIN7985248)

Target: Dihydrofolate Reductase (DHFR)

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Dihydrofolate Reductase Antikörper ist konjugiert mit Fluoro488

Applikation: Flow Cytometry (FACS)

Produktdetails anti-Dihydrofolate Reductase Antikörper (Fluoro488)

Bindungsspezifität: AA 2-187

Verwendungszweck: Anti-Dihydrofolate reductase (DHFR) Antibody Fluoro488 Conjugated

Spezifität: No cross reactivity with other proteins.

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Homologie: Human DHFR shares 90% amino acid (aa) sequence identity with both mouse,rat DHFR.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human DHFR recombinant protein (Position: V2-D187). Human DHFR shares 90% amino acid (aa) sequence identity with both mouse and rat DHFR.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Flow Cytometry, Optimal dilutions should be determined by end users.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Haltbarkeit: 12 months

Details zu Dihydrofolate Reductase

Target: Dihydrofolate Reductase (DHFR)

Andere Bezeichnung: DHFR

Hintergrund:

Background: Dihydrofolate reductase, or DHFR, is an enzyme that reduces dihydrofolic acid to tetrahydrofolic acid, using NADPH as electron donor, which can be converted to the kinds of tetrahydrofolate cofactors used in 1-carbon transfer chemistry. In humans, the DHFR enzyme is encoded by the DHFR gene. It is found in the q11→q22 region of chromosome 5. What's more, DHFR belongs to the dihydrofolate reductase family, and it converts dihydrofolate into tetrahydrofolate, a methyl group shuttle required for the de novo synthesis of purines, thymidylic acid, and certain amino acids. DHFR is the key enzyme in folate metabolism. In addition, DHFR catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis.

Gene Full Name: dihydrofolate reductase

Sequence Similarities: Belongs to the dihydrofolate reductase family.

Gen-ID: 1719

UniProt: P00374

Pathways: Mitotic G1-G1/S Phases