TdT Antikörper (AA 316-509) (Fluoro647)

Produktnummer ABIN7984885

Der Kaninchen Polyklonal anti-TdT Antikörper wird verwendet zum Nachweis von TdT in Proben von Human. Er wurde validiert für FACS.

Kurzübersicht für TdT Antikörper (AA 316-509) (Fluoro647) (ABIN7984885)

Target: TdT (DNTT) (Deoxynucleotidyltransferase, terminal (DNTT))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TdT Antikörper ist konjugiert mit Fluoro647

Applikation: Flow Cytometry (FACS)

Produktdetails anti-TdT Antikörper (Fluoro647)

Bindungsspezifität: AA 316-509

Verwendungszweck: Anti-TdT/DNTT Antibody Fluoro647 Conjugated

Spezifität: No cross reactivity with other proteins.

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Homologie: Human TdT shares 81% amino acid (aa) sequence identity with mouse TdT.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human TdT recombinant protein (Position: K316-A509). Human TdT shares 81% amino acid (aa) sequence identity with mouse TdT.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Flow Cytometry, Optimal dilutions should be determined by end users.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Haltbarkeit: 12 months

Details zu TdT

Target: TdT (DNTT) (Deoxynucleotidyltransferase, terminal (DNTT))

Andere Bezeichnung: DNTT

Hintergrund:

Background: Terminal Deoxynucleotidyl Transferase, also known as TDT and terminal transferase, is a unique DNA polymerase without template ion catalyzes the addition of deoxyribonucleotides onto the 3-prime-hydroxyl end of DNA primers. Its gene is mapped to the region 10q23-q24. And TDT cDNA contains an open reading frame of 1,530 basepairs corresponding to a protein containing 510 amino acids. TDT may be responsible for inserting nucleotides (N regions) at the V (H)-D and D-J (H) junctions of immunoglobulin genes. The enzyme is present in immature thymocytes, some bone marrow cells, transformed pre-B and pre-T cell lines, and leukemia cells. Additionally, TDT catalyses the addition of nucleotides to the 3' terminus of a DNA molecule. Unlike most DNA polymerases it does not require a template. The preferred substrate of this enzyme is a 3'-overhang, but it can also add nucleotides to blunt or recessed 3' ends. Cobalt is a necessary cofactor.

Gene Full Name: DNA nucleotidylexotransferase

Sequence Similarities: Belongs to the DNA polymerase type-X family.

Gen-ID: 1791

UniProt: P04053

Pathways: DNA Reparatur