CENPA Antikörper (AA 1-140) (HRP)

Produktnummer ABIN7978040

Der HRP-konjugierte Kaninchen Polyklonal anti-CENPA Antikörper (ABIN7978040) detektiert spezifisch CENPA in WB, ELISA und IHC. Dieser Antikörper reagiert spezifisch mit Proben aus Human und Ratte.

Kurzübersicht für CENPA Antikörper (AA 1-140) (HRP) (ABIN7978040)

Target: CENPA (Centromere Protein A (CENPA))

Reaktivität: Human, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CENPA Antikörper ist konjugiert mit HRP

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Produktdetails anti-CENPA Antikörper (HRP)

Bindungsspezifität: AA 1-140

Verwendungszweck: Anti-CENPA Antibody HRP Conjugated

Spezifität: No cross reactivity with other proteins.

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Homologie: Human CENPA shares 71.6% amino acid (aa) sequence identity with mouse CENPA.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human CENPA recombinant protein (Position: M1-G140). Human CENPA shares 71.6% amino acid (aa) sequence identity with mouse CENPA.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, Optimal dilutions should be determined by end users. Immunohistochemistry (Paraffin-embedded Section), Optimal dilutions should be determined by end users. ELISA, Optimal dilutions should be determined by end users.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4.

Lagerung: -20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Details zu CENPA

Target: CENPA (Centromere Protein A (CENPA))

Andere Bezeichnung: CENPA

Hintergrund:

Background: Centromeres are the differentiated chromosomal domains that specify the mitotic behavior of chromosomes. CENPA encodes a centromere protein which contains a histone H3 related histone fold domain that is required for targeting to the centromere. And CENPA is proposed to be a component of a modified nucleosome or nucleosome-like structure in which it replaces 1 or both copies of conventional histone H3 in the (H3-H4)2 tetrameric core of the nucleosome particle. Alternative splicing results in multiple transcript variants encoding distinct isoforms. In higher eukaryotes, the recruitment of CENP-A nucleosomes to existing centromeres is an epigenetic process, independent of the underlying DNA sequence. In S.pombe, de novo recruitment of the CENP-A to the centromere is believed to be controlled by centromeric heterochromatin surrounding the centromere, and by an RNAi mechanism. The RNAi is cut to form siRNA, this complexes with the protein Chp1, which then binds the centromeric heterochromatin. This helps recruit other proteins, ultimately resulting in a protein complex that forms cohesin between two sister chromatids at the centromeric heterochromatin. This cohesin is believed to be essential in replacing the centromere H3 with CENP-A. CENP-A is one of the epigenetic changes that is believed to distinguish centromeric DNA from other DNA. Once the CENP-A has been added, the centromere becomes self-propagating, and the surrounding heterochromatin/RNAi mechanism is no longer necessary.

Gene Full Name: centromere protein A

Sequence Similarities: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.

Gen-ID: 1058

UniProt: P49450

Pathways: Chromatin Binding, Maintenance of Protein Location