DDB2 Antikörper (AA 201-300)

Produktnummer ABIN747803

Produktdetails anti-DDB2 Antikörper

Target: DDB2 (Damage-Specific DNA Binding Protein 2, 48kDa (DDB2))

Bindungsspezifität: AA 201-300

Reaktivität: Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser DDB2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Spezifität: This antibody may have a minor secondary cross-reactivity to TNFAIP3-interacting protein 2 due to a 63 % non-sequential sequence similarity in the immunogen region.

Kreuzreaktivität: Maus, Ratte

Homologie: Human,Cow,Horse,Rabbit

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human DDB2

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit: 12 months

Details zu DDB2

Target: DDB2 (Damage-Specific DNA Binding Protein 2, 48kDa (DDB2))

Andere Bezeichnung: DDB2 (DDB2 Produkte)

Synonyme: 2610043A19Rik antikoerper, si:dkey-45f10.3 antikoerper, DDBB antikoerper, UV-DDB2 antikoerper, damage specific DNA binding protein 2 antikoerper, damage-specific DNA binding protein 2 antikoerper, DDB2 antikoerper, ddb2 antikoerper, Ddb2 antikoerper

Hintergrund:

Synonyms: DDBB, UV-DDB2, DNA damage-binding protein 2, DDB p48 subunit, Damage-specific DNA-binding protein 2, UV-damaged DNA-binding protein 2, UV-DDB 2, DDB2

Background: Required for DNA repair. Binds to DDB1 to form the UV-damaged DNA-binding protein complex (the UV-DDB complex). The UV-DDB complex may recognize UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair. The UV-DDB complex preferentially binds to cyclobutane pyrimidine dimers (CPD), 6-4 photoproducts (6-4 PP), apurinic sites and short mismatches. Also appears to function as the substrate recognition module for the DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex DDB1-CUL4-ROC1 (also known as CUL4-DDB-ROC1 and CUL4-DDB-RBX1). The DDB1-CUL4-ROC1 complex may ubiquitinate histone H2A, histone H3 and histone H4 at sites of UV-induced DNA damage. The ubiquitination of histones may facilitate their removal from the nucleosome and promote subsequent DNA repair. The DDB1-CUL4-ROC1 complex also ubiquitinates XPC, which may enhance DNA-binding by XPC and promote NER. Isoform D1 and isoform D2 inhibit UV-damaged DNA repair.

Gen-ID: 1643

UniProt: Q92466

Pathways: DNA Reparatur