DDB2 Antikörper (AA 201-300)

Produktnummer ABIN747803

Der Kaninchen Polyklonal anti-DDB2 Antikörper wird verwendet zum Nachweis von DDB2 in Proben von Human. Er wurde validiert für WB, IF (p), IF (cc), IHC (fro) und IHC (p).

Kurzübersicht für DDB2 Antikörper (AA 201-300) (ABIN747803)

Target: DDB2 (Damage-Specific DNA Binding Protein 2, 48kDa (DDB2))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser DDB2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-DDB2 Antikörper

Bindungsspezifität: AA 201-300

Verwendungszweck: DDB2 Polyclonal Antibody

Spezifität: This antibody may have a minor secondary cross-reactivity to TNFAIP3-interacting protein 2 due to a 63 % non-sequential sequence similarity in the immunogen region.

Kreuzreaktivität: Maus, Ratte

Homologie: Human,Cow,Horse,Rabbit

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human DDB2

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB(1:300-5000),

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit: 12 months

Details zu DDB2

Target: DDB2 (Damage-Specific DNA Binding Protein 2, 48kDa (DDB2))

Andere Bezeichnung: DDB2

Hintergrund:

Synonyms: DDBB, UV-DDB2, DNA damage-binding protein 2, DDB p48 subunit, Damage-specific DNA-binding protein 2, UV-damaged DNA-binding protein 2, UV-DDB 2, DDB2

Background: Required for DNA repair. Binds to DDB1 to form the UV-damaged DNA-binding protein complex (the UV-DDB complex). The UV-DDB complex may recognize UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair. The UV-DDB complex preferentially binds to cyclobutane pyrimidine dimers (CPD), 6-4 photoproducts (6-4 PP), apurinic sites and short mismatches. Also appears to function as the substrate recognition module for the DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex DDB1-CUL4-ROC1 (also known as CUL4-DDB-ROC1 and CUL4-DDB-RBX1). The DDB1-CUL4-ROC1 complex may ubiquitinate histone H2A, histone H3 and histone H4 at sites of UV-induced DNA damage. The ubiquitination of histones may facilitate their removal from the nucleosome and promote subsequent DNA repair. The DDB1-CUL4-ROC1 complex also ubiquitinates XPC, which may enhance DNA-binding by XPC and promote NER. Isoform D1 and isoform D2 inhibit UV-damaged DNA repair.

Gen-ID: 1643

UniProt: Q92466

Pathways: DNA Reparatur