Beclin 1 Antikörper (AA 1-354)

Produktnummer ABIN7972552

Der Maus Monoklonal anti-Beclin 1 Antikörper wird verwendet zum Nachweis von Beclin 1 in Proben von Human, Maus und Ratte. Er wurde validiert für WB, IHC, IF, FACS und ICC.

Kurzübersicht für Beclin 1 Antikörper (AA 1-354) (ABIN7972552)

Target: Beclin 1 (BECN1) (Beclin 1, Autophagy Related (BECN1))

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Beclin 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Güteklasse: Carrier-free

Klon: 2D12A3

Produktdetails anti-Beclin 1 Antikörper

Bindungsspezifität: AA 1-354

Verwendungszweck: Anti-Beclin 1 Antibody (monoclonal, 2D12A3)

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Homologie: Human Beclin 1 shares 97% amino acid (aa) sequence identity with both mouse,rat Beclin 1.

Produktmerkmale: Anti-Beclin 1 Antibody.(monoclonal, 2D12A3)-2. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human Beclin 1 recombinant protein (Position: M1-S354). Human Beclin 1 shares 97% amino acid (aa) sequence identity with both mouse and rat Beclin 1.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Details zu Beclin 1

Target: Beclin 1 (BECN1) (Beclin 1, Autophagy Related (BECN1))

Andere Bezeichnung: BECN1

Hintergrund:

Background: Beclin-1, also known as also known as ATG6 or VPS30 is a protein that in humans is encoded by the BECN1 gene. Beclin-1 and its binding partner class III phosphoinositide 3-kinase (PI3K), also named Vps34, are required for the initiation of the formation of the autophagosome in autophagy. This gene participates in the regulation of autophagy and has an important role in development, tumorigenesis, and neurodegeneration. Schizophrenia is associated with low levels of Beclin-1 in the hippocampus of the affected which causes diminished autophagywhich in turn results in increased neuronal cell death. It has been found that beclin-1 can promote autophagy in autophagy-defective yeast with a targeted disruption of apg6/vps30, and in human MCF7 breast carcinoma cells.

Gene Full Name: beclin 1

Molekulargewicht: 52-60 kDa

Gen-ID: 8678

UniProt: Q14457

Pathways: Autophagie