BIM Antikörper (AA 18-186)

Produktnummer ABIN7972474

Der Kaninchen Polyklonal anti-BIM Antikörper wird verwendet zum Nachweis von BIM in Proben von Maus. Er wurde validiert für WB, ELISA und FACS.

Kurzübersicht für BIM Antikörper (AA 18-186) (ABIN7972474)

Target: BIM (BCL2L11) (BCL2-Like 11 (Apoptosis Facilitator) (BCL2L11))

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser BIM Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Güteklasse: Carrier-free

Produktdetails anti-BIM Antikörper

Bindungsspezifität: AA 18-186

Verwendungszweck: Anti-Bim/Bcl2l11 Antibody

Kreuzreaktivität (Details): No cross reactivity with other proteins.

Homologie: Mouse Bcl2l11 shares 86.7%,98.8% amino acid (aa) sequence identity with human,rat Bcl2l11,respectively.

Produktmerkmale: Anti-Bim/Bcl2l11 Antibody. Tested in WB, Flow Cytometry, ELISA applications. This antibody reacts with Mouse.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived mouse Bim/Bcl2l11 recombinant protein (Position: Q18-R186). Mouse Bcl2l11 shares 86.7% and 98.8% amino acid (aa) sequence identity with human and rat Bcl2l11, respectively.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Mouse Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Mouse ELISA, 0.1-0.5 μg/mL, -

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Details zu BIM

Target: BIM (BCL2L11) (BCL2-Like 11 (Apoptosis Facilitator) (BCL2L11))

Andere Bezeichnung: Bcl2l11

Hintergrund:

Background: Bcl-2-like protein 11, commonly called BIM (Bcl-2?Interacting?Mediator of cell death), is a?protein?that in humans is encoded by the?BCL2L11?gene. BCL2 is a prototypic mammalian regulator of cell death. Certain members of the BCL2 family inhibit apoptosis, while other members facilitate this physiologic process of cell death. By screening a cDNA expression library using recombinant BCL2 as bait, O'Connor et al. isolated a small novel protein, which they referred to as BIM, with a 9-amino acid BH3 motif shared by most BCL2 homologs. BIM provokes apoptosis, and the BH3 region is required for BCL2 binding and for most of its cytotoxicity.

Gene Full Name: BCL2 like 11

Molekulargewicht: 22 kDa

Gen-ID: 12125

UniProt: O54918

Pathways: PI3K-Akt Signalweg, Neurotrophin Signalübertragung, Tube Formation, Positive Regulation of Endopeptidase Activity