PUMA Antikörper (C-Term) (Biotin)

Produktnummer ABIN7972233

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch PUMA in WB, ELISA und IHC. Es zeigt Reaktivität gegenüber Proben von Human, Ratte und Maus.

Kurzübersicht für PUMA Antikörper (C-Term) (Biotin) (ABIN7972233)

Target: PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PUMA Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Produktdetails anti-PUMA Antikörper (Biotin)

Bindungsspezifität: C-Term

Verwendungszweck: Anti-PUMA/BBC3 Antibody Biotin Conjugated

Spezifität: No cross reactivity with other proteins.

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Homologie: identical to the related rat,mouse sequences.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human PUMA, identical to the related rat and mouse sequences.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, Optimal dilutions should be determined by end users. Immunohistochemistry (Paraffin-embedded Section), Optimal dilutions should be determined by end users. ELISA, Optimal dilutions should be determined by end users.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Details zu PUMA

Target: PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))

Andere Bezeichnung: BBC3

Hintergrund:

Background: The p53 upregulated modulator of apoptosis, or PUMA, is a pro-apoptotic member of the Bcl-2 protein family. The PUMA gene is located at 19q. PUMA transcript is contained within 4 exons, with the presumptive initiation codon in exon 2. The predicted 193-amino acid PUMA protein shares 91 % amino acid identity with the murine sequence. Bcl-2 family members can form hetero- or homodimers, and they act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The expression of PUMA is regulated by the tumor suppressor p53, and PUMA has been shown to be involved in p53-mediated apoptosis. Additionally, PUMA encodes 2 BH3 domain-containing proteins, PUMA-alpha and PUMA-beta, that are produced through the use of an alternative first exon and are induced in cells following p53 activation. Furthermore, PUMA couples the nuclear and cytoplasmic proapoptotic functions of p53.

Gene Full Name: BCL2 binding component 3

Sequence Similarities: Belongs to the Bcl-2 family.

Gen-ID: 27113

UniProt: Q9BXH1

Pathways: p53 Signalweg, Positive Regulation of Endopeptidase Activity