YTHDF2 Antikörper (C-Term)

Produktnummer ABIN7938549

Der Kaninchen Polyklonal anti-YTHDF2 Antikörper wird verwendet zum Nachweis von YTHDF2 in Proben von Human. Er wurde validiert für WB.

Kurzübersicht für YTHDF2 Antikörper (C-Term) (ABIN7938549)

Target: YTHDF2 (YTH Domain Family, Member 2 (YTHDF2))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser YTHDF2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-YTHDF2 Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: YTHDF2 antibody (pAb)

Aufreinigung: Affinity Purified

Immunogen: This YTHDF2 antibody was raised against a fusion protein with His-tags at the C-terminus.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 1 - 4 μg/mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: Purified IgG in PBS, 30 % glycerol, 0.035 % Azide. Sodium Azide is highly toxic.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Lagerung: -20 °C

Informationen zur Lagerung: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Haltbarkeit: 24 months

Details zu YTHDF2

Target: YTHDF2 (YTH Domain Family, Member 2 (YTHDF2))

Andere Bezeichnung: YTHDF2

Hintergrund:

Background_Plain: YTHDF2 (YT521-B homology domain family 2, also known as CAHL, HGRG8, NY-REN-2) is a member of the YTH (YT521-B homology) superfamily containing YTH domain. Human YTH domain family proteins include three members, YTHDF1-3, which mainly localized in the cytoplasm. YTHDF proteins contain a highly conserved single-stranded RNA-binding domain which can specifically recognize and bind m6A-containing RNAs. An important role of YTHDF2is in regulating mRNA stability. YTHDF2 recognizes many m6A-containing mRNAs that are not being actively translated and recruits them to cytoplasmic processing (P) bodies for degradation. YTHDF2 preserves 5'UTR methylation of stress- induced transcripts and promotes cap-independent mRNA translation by competitive binding and limiting the m6A 'eraser' FTO from demethylation. N6-methylated adenine (m6A) is prevalently present in nearly all RNA types and can be found in all organisms from bacteria to humans. It preferentially appears around stop codons and within long internal exons in mammalian messager RNAs. m6A plays an important role in the efficiency of mRNA splicing, processing, translation efficiency, editing and mRNA stability. m6A also takes place in other RNA molecules, such as primary miRNA (pri-miRNAs).

Aliases: YTH N6-Methyladenosine RNA Binding Protein 2, HGRG8

Molekulargewicht: 70 kDa

NCBI Accession: NP_057342