Rekombinanter UBE2S Antikörper

Produktnummer ABIN7938534

Der Kaninchen Monoklonal anti-UBE2S Antikörper (Klon 6D18) (ABIN7938534) detektiert spezifisch UBE2S in WB und IHC. Dieser Antikörper reagiert spezifisch mit Proben aus Human und Maus.

Kurzübersicht für Rekombinanter UBE2S Antikörper (ABIN7938534)

Target: UBE2S (Ubiquitin-Conjugating Enzyme E2S (UBE2S))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser UBE2S Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Klon: 6D18

Produktdetails anti-UBE2S Antikörper

Verwendungszweck: UBE2S antibody (rAb), 100 μg (Clone 6D18)

Aufreinigung: Protein A Chromatography

Immunogen: This antibody was raised against the UBE2S fusion protein.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 1:5000-1:50000 dilution IHC: 1:2000-1:10000 dilution

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: Purified IgG in PBS, pH 7.3, with 50 % glycerol and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Lagerung: -20 °C

Informationen zur Lagerung: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Haltbarkeit: 24 months

Details zu UBE2S

Target: UBE2S (Ubiquitin-Conjugating Enzyme E2S (UBE2S))

Andere Bezeichnung: UBE2S

Hintergrund:

Background_Plain: UBE2S (Ubiquitin-conjugating enzyme E2 S) also known as E2-EPF, E2EPF and EPF5. UBE2S is a member of the E2 ubiquitin-conjugating enzyme family which is required for post-replicative DNA damage repair and plays an important role in various cellular processes. UBE2S can accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes 'Lys-11'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by specifically elongating 'Lys-11'-linked polyubiquitin chains initiated by the E2 enzyme UBE2C/UBCH10 on APC/C substrates, enhancing the degradation of APC/C substrates by the proteasome and promoting mitotic exit. Also acts by elongating ubiquitin chains initiated by the E2 enzyme UBE2D1/UBCH5 in vitro, it is however unclear whether UBE2D1/UBCH5 acts as an E2 enzyme for the APC/C in vivo. Also involved in ubiquitination and subsequent degradation of VHL, resulting in an accumulation of HIF1A. In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, except 'Lys-48'-linked polyubiquitination.

Aliases: Ubiquitin conjugating enzyme E2 S, E2-EPF, E2EPF, EPF5

Molekulargewicht: 24 kDa

Pathways: Ubiquitin Proteasome Pathway