TET1 Antikörper (AA 1682-1914)

Produktnummer ABIN5563987

Der Ratte Monoklonal anti-TET1 Antikörper wird verwendet zum Nachweis von TET1 in Proben von Human und Maus. Er wurde validiert für WB, IF, IP, ICC und ChIP-seq.

Kurzübersicht für TET1 Antikörper (AA 1682-1914) (ABIN5563987)

Target: TET1 (Tet Methylcytosine Dioxygenase 1 (TET1))

Reaktivität: Human, Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser TET1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC), ChIP DNA-Sequencing (ChIP-seq)

Klon: 5D6

Produktdetails anti-TET1 Antikörper

Bindungsspezifität: AA 1682-1914

Verwendungszweck: Tet1 antibody (mAb) (Clone 5D6)

Aufreinigung: Protein G Chromatography

Immunogen: This antibody was raised against a recombinant protein corresponding amino acids 1682-1914 of mouse Tet1.

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: ChIP-Seq: 4 µL per ChIP This antibody is also available as an AbFlex engineered recombinant antibody. For details on the corresponding AbFlex Recombinant Antibody, see Catalog No. 91171.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Lagerung: -20 °C

Informationen zur Lagerung: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Haltbarkeit: 24 months

Details zu TET1

Target: TET1 (Tet Methylcytosine Dioxygenase 1 (TET1))

Andere Bezeichnung: Tet1

Hintergrund:

Background_Plain: Tet1 (Ten-Eleven Translocation-1, methylcytosine dioxygenase) is a protein that catalyzes the conversion of the modified genomic base 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC) which can lead to cytosine demethylation by either further oxidation via Tet proteins to 5-formylcytosine (5fC) and 5-carboxycytosine (5caC) or deamination into 5-hydroxymethyluracil (5hmU) and then subsequent replacement by unmethylated cytosine by the base excision repair system. Methylation at the C5 position of cytosine bases is an epigenetic modification of the mammalian genome which plays an important role in transcriptional regulation. Tet1 preferentially binds to CpG-rich sequences at promoters of both transcriptionally active and polycomb-repressed genes. By controlling the levels of 5mC and 5hmC at gene promoters, it may regulate the gene expression silencing induced by cytosine methylation. May have a dual function by also repressing the expression of a subset of genes through recruitment of transcriptional repressors to promoters. Involved in the balance between pluripotency and lineage commitment of cells, it plays a role in embryonic stem cells maintenance and inner cell mass cell specification.

Aliases: LCX, Cxxc6, AA517754, BB001228, mKIAA1676, D10Ertd17e, 2510010B09Rik

Molekulargewicht: 215 kDa

NCBI Accession: NP_001240786

Pathways: Stem Cell Maintenance, Warburg Effekt