Histone 3 Antikörper (H3K56ac)

Produktnummer ABIN7938240

Der Kaninchen Polyklonal anti-Histone 3 Antikörper (ABIN7938240) detektiert spezifisch Histone 3 in WB, ChIP, DB, ChIP-seq und CUT&Tag. Dieser Antikörper reagiert spezifisch mit Proben aus Human und Saccharomyces cerevisiae.

Kurzübersicht für Histone 3 Antikörper (H3K56ac) (ABIN7938240)

Target: Histone 3 (H3) (Histone H3 (H3))

Reaktivität: Human, Saccharomyces cerevisiae

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Histone 3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), ChIP DNA-Sequencing (ChIP-seq), Cleavage Under Targets and Tagmentation (CUT&Tag)

Produktdetails anti-Histone 3 Antikörper

Bindungsspezifität: H3K56ac

Verwendungszweck: Histone H3K56ac antibody (pAb)

Aufreinigung: Affinity Purified

Immunogen: This Histone H3 acetyl Lys56 antibody was raised against peptide containing acetyl-Lys56 of yeast histone H3.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ChIP: 3 - 5 µL per ChIP ChIP-Seq: 10 µL each WB: 1:2,500 - 1:5,000 dilution CUT&Tag: 1-2 µL per 50 µL reaction For Histone H3K56ac, we also offer AbFlex Histone H3K56ac Recombinant Antibody (rAb). For details, see Catalog No. 91127.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: Purified rabbit IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Lagerung: -20 °C

Informationen zur Lagerung: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Haltbarkeit: 24 months

Details zu Histone 3

Target: Histone 3 (H3) (Histone H3 (H3))

Andere Bezeichnung: Histone H3

Hintergrund: Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points, it is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, they play a major role in regulating gene expression. Lysine N-e-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in chromatin remodeling and in the regulation of gene expression in various cellular functions. Acetylation of histone H3 occurs at several different lysine positions in the histone tail, and is performed by Histone Acetyltransferases (HATs) such as CBP/p300. Acetylation of histones is often associated with transcriptional activation. Histone H3 Lys56 acetylation occurs normally during S phase, but disappears in G2. This modification persists in presence of DNA damage and also plays a role in nucleosome assembly. Rtt109 was shown to be the major histone acetyltransferase (HAT) for Lys56 acetylation.

Molekulargewicht: 17 kDa

NCBI Accession: NP_009564