Histone 3 Antikörper (H3K36me2)

Produktnummer ABIN7938235

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch Histone 3 in WB, IF, ChIP, ICC, DB, ChIP-seq und CUT&Tag. Es zeigt Reaktivität gegenüber Proben von Human und Saccharomyces cerevisiae.

Kurzübersicht für Histone 3 Antikörper (H3K36me2) (ABIN7938235)

Target: Histone 3 (H3) (Histone H3 (H3))

Reaktivität: Human, Saccharomyces cerevisiae

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Histone 3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Dot Blot (DB), ChIP DNA-Sequencing (ChIP-seq), Cleavage Under Targets and Tagmentation (CUT&Tag)

Produktdetails anti-Histone 3 Antikörper

Bindungsspezifität: H3K36me2

Verwendungszweck: Histone H3K36me2 antibody (pAb)

Aufreinigung: Unpurified

Immunogen: This Histone H3 dimethyl Lys36 antibody was raised against a peptide including dimethyl-lysine 36 of histone H3.

Anwendungsinformationen

Applikationshinweise: ChIP: 3 - 5 µL per ChIP ChIP-Seq: 10 µL each ICC/IF: 1:250 - 1:1,000 dilution WB: 1:2500 - 1:5,000 dilution CUT&Tag: 1-2 µL per 50 µL reaction

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: Rabbit serum containing 30 % glycerol and 0.035 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Lagerung: -20 °C

Informationen zur Lagerung: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Haltbarkeit: 24 months

Details zu Histone 3

Target: Histone 3 (H3) (Histone H3 (H3))

Andere Bezeichnung: Histone H3

Hintergrund: Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, these modifications play a major role in regulating gene expression. The methylation of histones can occur on two different residues: arginine or lysine. Histone methylation can be associated with transcriptional activation or repression, depending on the methylated residue. Histone H3 is methylated at lysine 36 by the Set2 (yeast) and NSD1 (mammals) methyltransferases. Dimethylation of lysine 36 of histone H3 is involved with transcriptional elongation by RNA pol II holoenzyme and is a marker of transcribed genes.

Molekulargewicht: 17 kDa

NCBI Accession: NP_003522