Histone H2A Antikörper (C-Term)

Produktnummer ABIN7938170

Der Kaninchen Polyklonal anti-Histone H2A Antikörper wird verwendet zum Nachweis von Histone H2A in Proben von Human. Er wurde validiert für WB, IF, DB, ChIP und ChIP-seq.

Kurzübersicht für Histone H2A Antikörper (C-Term) (ABIN7938170)

Target: Histone H2A

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Histone H2A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Dot Blot (DB), Chromatin Immunoprecipitation (ChIP), ChIP DNA-Sequencing (ChIP-seq)

Produktdetails anti-Histone H2A Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: Histone H2A.Z antibody (pAb) (H2AZ)

Aufreinigung: Unpurified

Immunogen: This Histone H2A.Z antibody was raised against a peptide derived from the C-terminus of human histone H2A.Z.

Anwendungsinformationen

Applikationshinweise: ChIP: 2 - 10 µL per ChIP ChIP-Seq: 5 µL each WB*: 1:1,000 - 1:5,000 dilution This histone variant can be perturbed by excessive washing steps prior to fixation. Cells should be washed into serum-free culture medium (NOT PBS!) prior to fixation. *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: Rabbit serum containing 30 % glycerol and 0.035 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Lagerung: -20 °C

Informationen zur Lagerung: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Haltbarkeit: 24 months

Details zu Histone H2A

Target: Histone H2A

Andere Bezeichnung: Histone H2A.

Hintergrund: The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points, it is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, they play a major role in regulating gene expression. Histone H2A.Z (H2AZ, H2AFZ) is a histone H2A variant, a protein similar to canonical H2A but with different molecular identity and unique functions. H2A.Z is highly conserved during evolution. It plays an important role in basic cellular mechanisms such as gene activation, chromosome segregation, heterochromatic silencing and progression through the cell cycle. H2A.Z is acetylated at multiple lysine residues in its amino terminus, which may enable H2A.Z to function as an insulator of chromatin domains.

Molekulargewicht: 14 kDa

NCBI Accession: NP_002097