Rekombinanter Histone H2A Antikörper (H2A.XS139ph)

Produktnummer ABIN7938169

Dieser Kaninchen Monoklonal Antikörper detektiert spezifisch Histone H2A in WB und ICC. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für Rekombinanter Histone H2A Antikörper (H2A.XS139ph) (ABIN7938169)

Target: Histone H2A

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser Histone H2A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunocytochemistry (ICC)

Klon: RM224

Produktdetails anti-Histone H2A Antikörper

Bindungsspezifität: H2A.XS139ph

Verwendungszweck: Histone H2A.XS139ph antibody (rAb) (H2AX) (Clone RM224)

Aufreinigung: Protein A Chromatography

Immunogen: A phospho-peptide corresponding to Phospho-Histone H2AX (Ser139).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 0.5 - 2 μg/mL ICC: 0.5 - 2 μg/mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: PBS with 50 % glycerol, 1 % BSA, and 0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Lagerung: -20 °C

Informationen zur Lagerung: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Haltbarkeit: 24 months

Details zu Histone H2A

Target: Histone H2A

Andere Bezeichnung: Histone H2A.

Hintergrund: Histone H2AX phospho Ser139 (H2AX, H2A histone family member X) replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries that require DNA as a template. Histones thereby play a central role in transcriptional regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called the histone code, and nucleosome remodeling. Histone H2AX is required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation, and for efficient repair of DNA double-strand breaks (DSBs), specifically when modified by C-terminal phosphorylation. RM224 reacts to Histone H2A.X only when phosphorylated at serine 139. No cross reactivity with other phosphorylated histones.

NCBI Accession: NP_002096