Histone H2A Antikörper (AA 122-129)

Produktnummer ABIN7938167

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch Histone H2A in WB und DB. Es zeigt Reaktivität gegenüber Proben von Human und Maus.

Kurzübersicht für Histone H2A Antikörper (AA 122-129) (ABIN7938167)

Target: Histone H2A

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Histone H2A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Dot Blot (DB)

Produktdetails anti-Histone H2A Antikörper

Bindungsspezifität: AA 122-129

Verwendungszweck: Histone H2A.J antibody (pAb)

Aufreinigung: Affinity Purified

Immunogen: This antibody was raised against a peptide comprising amino acids 122-129 of human Histone H2A.J.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB*: 1:500 - 1:2500 dilution DB: 1:1000 dilution *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Lagerung: -20 °C

Informationen zur Lagerung: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Haltbarkeit: 24 months

Details zu Histone H2A

Target: Histone H2A

Andere Bezeichnung: Histone H2A.

Hintergrund: Histone H2A is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points, and is responsible for establishing higher-order chromatin structure. Histone H2A.J is a histone H2A variant, a protein similar to canonical H2A but with different molecular identity and unique functions. Histone H2A.J, which is found only in mammals, is shown to be associated with senescence, accumulating in senescent fibroblasts and promoting expression of inflammatory genes. Therefore, H2A.J may contribute to chronic inflammation and age-related diseases.

NCBI Accession: NP_808760