Histone H2A Antikörper (H2AK119ub)

Produktnummer ABIN7938165

Der Kaninchen Polyklonal anti-Histone H2A Antikörper wird verwendet zum Nachweis von Histone H2A in Proben von Human. Er wurde validiert für WB und DB.

Kurzübersicht für Histone H2A Antikörper (H2AK119ub) (ABIN7938165)

Target: Histone H2A

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Histone H2A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Dot Blot (DB)

Produktdetails anti-Histone H2A Antikörper

Bindungsspezifität: H2AK119ub

Verwendungszweck: Histone H2AK119ub antibody (pAb)

Aufreinigung: Affinity Purified

Immunogen: This antibody was raised against a branched peptide corresponding to the ubiquitin conjugation site at Lys119 of human Histone H2A.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: DB: 1:1000 WB*: 1:1000 - 1:2000 dilution *Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot. For optimal results, we recommend the addition of 0.05 % Tween-20 to all blocking solutions to reduce background. Individual optimization may be required.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol, and 0.035 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Lagerung: -20 °C

Informationen zur Lagerung: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Haltbarkeit: 24 months

Details zu Histone H2A

Target: Histone H2A

Hintergrund: In humans, attachment of ubiquitin to Histone protein 2A (H2A) on lysine residue 119 (H2AK119Ub) is one of the most prevalent histone marks, and is estimated to occur on 5-15 % of histones genome-wide4. In general, H2AK119Ub is characteristic of repressed developmental genes, and is also tightly linked to levels of other histone marks such as lysine methylation and acetylation. H2AK119Ub is attached to histones by the Really Interesting New Gene (RING) ubiquitin E3-ligase Polycomb Repressive Complex 1 (PRC1), and removed by the Polycomb Repressive-Deubiquitinase (PR-DUB) complex, which plays a vital role in regulating H2AK119Ub histone marks, and mutations in human PR-DUB genes lead to a spectrum of malignancies.

Molekulargewicht: 23 kDa

NCBI Accession: NP_003508